侵染陕西洋葱的胡葱黄条病毒基因组全序列分析  

Full-length Sequence Analysis of Shallot Yellow Stripe Virus from Onion in Shaanxi Province

作　　者：罗朝鹏[1] 林林[1] 陈炯[2] 吴云锋[1] 陈剑平[2] 

机构地区：[1]西北农林科技大学植物保护学院,陕西杨凌712100 [2]浙江省农业科学院病毒学与生物技术研究所,浙江杭州310021

出　　处：《病毒学报》2007年第2期157-160,共4页Chinese Journal of Virology

基　　金：浙江省自然科学基金重点项目(批准号:ZA0207);国家自然科学基金项目(3020008);国家杰出青年科学基金项目(30225031)

摘　　要：The nucleotide sequence of shallot yellow stripe virus from onion(SYSV-O)in Shaanxi Province of China was described.It was 10427 nucleotides(nt)in length excluding the poly(A)tail and was predicted to encode a polyprotein of 3340 amino acids(aa)with a calculated molecular weight of 379.7kD.SYSV was most closely related to onion yellow dwarf virus(OYDV)and resembled it in having a much larger P3 protein than other species in the genus.SYSV-O shared 93.2% nt identity to the published sequence of a welsh onion isolate of SYSV(SYSV-ZQ2).The two isolates differed in NIb-CP cleavage site,that of SYSV-O was Q/V but Q/A in SYSV-ZQ2.Nucleotide identity of coat protein genes amongst all SYSV sequences ranged from 87.3%-84.1%.Phylogenetic tree analysis based on the 3’-terminal nucleotide sequences of SYSV isolates confirmed the existence of four distinct groups that were partially related to geographical origin and host plant species.The nucleotide sequence of shallot yellow stripe virus from onion （SYSV-O） in Shaanxi Province of China was described. It was 10427 nucleotides （nt） in length excluding the poly（A） tail and was predicted to encode a polyprotein of 3340 amino acids （aa） with a calculated molecular weight of 379.7kD. SYSV was most closely related to onion yellow dwarf virus （OYDV） and resembled it in having a much larger P3 protein than other species in the genus. SYSV-O shared 93.2% nt identity to the published sequence of a welsh onion isolate of SYSV （SYSV-ZQ2）. The two isolates differed in NIb-CP cleavage site, that of SYSV-O was Q/V but Q/A in SYSV-ZQ2. Nucleotide identity of coat protein genes amongst all SYSV sequences ranged from 87. 3%-84.1%. Phylogenetic tree analysis based on the 3′-terminal nucleotide sequences of SYSV isolates confirmed the existence of four distinct groups that were partially related to geographical origin and host plant species.

关 键 词：胡葱黄条病毒 洋葱分离物 基因组全序列 序列分析 系统进化树 

分 类 号：S432.41[农业科学—植物病理学]