腈水合酶的催化反应动力学与失活动力学研究  

STUDY ON ENZYME CATALYTIC KINETICS AND DE-ACTIVE DYNAMICS OF NITRILE HYDRATASE

作　　者：邱峰[1] 李志东[1] 李娜[1] 郑佳[1] 

机构地区：[1]辽宁石油化工大学环境工程系,辽宁抚顺113001

出　　处：《矿冶》2007年第1期50-53,21,共5页Mining And Metallurgy

摘　　要：在以丙烯腈为原料,微生物转化生产丙烯酰胺的过程中.酶催化反应是过程的关键。为了了解酶催化的动力学,本文以自由细胞的酶为催化剂,进行了腈水合酶的反应动力学和失活动力学的研究。首先研究了菌体浓度、温度、pH值、丙烯腈浓度、丙烯酰胺浓度等对腈水合酶催化反应速度的影响。结果表明,在这些因素中,温度和丙烯酰胺浓度是最主要的影响因素。经过对不同温度下的反应速度的研究,得到腈水合酶水合反应的活化能Ea为60.87kJ/mol,酶失活反应的活化能为89.36kJ/mol。The hydration reaction by microbial method is the key of the procedure for acrylamide production from acrylonltrile. The paper studies the enzyme catalytic kinetics and deactivation kinetics of nitrile hydratase in the type of free cell. Firstly the effects of the temperature, pH and the concentration of acrylonitrile, acrylamide and cell on the activity of nitrile hydratase are investigated. The results show that the temperature and the concentration of acrylamide are the most important factors, the activation energy of the hydration reaction and the deactivation energy for nitrile hydratase are 60.87kJ/mol, 89.36 kJ/mol respectively.

关 键 词：腈水合酶 催化动力学 失活动力学 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]