云南省德宏州冬作马铃薯晚疫病菌表型特征研究  

Phenotype characteristics of Phytophthora infestans on winter-growing potato in Dehong district of Yunnan province

作　　者：杨素祥[1] 李灿辉[1] 郝大海[1] 顾中量 陈善娜[3] 

机构地区：[1]云南师范大学生命科学学院,昆明650092 [2]云南省德宏州农业科学研究所,潞西678400 [3]云南大学生命科学学院,昆明650031

出　　处：《植物病理学报》2007年第1期18-24,共7页Acta Phytopathologica Sinica

基　　金：云南省"十五"科技攻关计划资助项目(2002NG09)

摘　　要：2003～2005年，从云南省德宏州新兴冬作马铃薯主产区采集的马铃薯晚疫病病样中分离纯化到86株致病疫霉菌，对其代表性菌株的表型特征进行了较系统的研究。交配型实验结果表明：所测定的62个菌株都属于A1交配型，未发现A2交配型或自育型菌株。毒性基因鉴定、监测及生理小种组成分析显示：德宏州存在所有已知可鉴定的毒性基因，而且生理小种组成非常复杂；所测定的66个菌株可区分出36个生理小种，其中，出现频率最高的小种为1，2，3，4，5，6，7，8，9，10，11，占被测菌株的18．18％，在主产区潞西县和盈江县均有分布。对60个菌株的甲霜灵抗性测定结果表明：56．67％的菌株表现为敏感，25．00％的菌株中抗，18．33％的菌株对甲霜灵有抗性。本文对此实验研究结果作了初步的分析和讨论。The total of 86 isolates of Phytophthora infestans were collected from newly developed winter-growing potatoes in Dehong district of Yunnan province during 2003-2005. The phenotype characteristics including mating type, virulence spectrum, physiological race structure, and response to metalaxyl were investigated systematically. The results showed that 62 isolates tested were all subjected to A1 mating type, and no A2 mating type or self-fertifity isolate were found. The virulence detection, monitoring and further analysis of physiological race structure demonstrated that all known R gene of potato could be overcome by corresponding virulence gene both in the artificial inoculation experiments and field monitoring trial of late blight differentials. The virulence component of race and its structure in Dehong were complicated as well. Among 66 isolates tested, 36 races could be distinguished with different virulence spectrum, and superior race of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 was most common in Dehong with the frequency of 18.18%. Response test to metalaxyl showed that, out of 60 isolates tested, 56.67% were sensitive, 25.00% intermediate resistant, and 18.33% resistant to metalaxyl. The results were preliminary analyzed and discussed.

关 键 词：马铃薯 致病疫霉 交配型 生理小种 甲霜灵抗性 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]