酒类酒球菌SD-2a高密度培养的研究  

Research on High-cell-density Cultivation of Oenococcus oeni SD-2a

作　　者：王华[1] 罗华[1] 黄科[1] 

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100

出　　处：《酿酒科技》2007年第4期69-72,共4页Liquor-Making Science & Technology

基　　金：科技部科技成果重点推广项目(2004EC000317)

摘　　要：对酒类酒球菌SD-2a液体培养条件和半连续高密度培养进行了研究,研究结果表明,酒类酒球菌SD-2a的适宜培养条件为:培养温度25℃,接种量5%,pH值4.8。在培养过程中,每4h添加一次CaCO3溶液,调整pH值至最适值。并结合优化培养条件,对酒类酒球菌SD-2a进行半连续法高密度培养,使其菌体密度达到了6.5×1010cfu/mL,比二次培养前提高了近10倍。The high-cell-density cultivation of Oenococcus oeni SD-2a and semi-continuous way was researched. The results showed that the optimal culturing conditions were as follows： culture temperature 25℃ ,pH4.8,inoculation size 5 %. Adjusting the pH value by using CaCO3 during culturing to obtain the optimal pH value. With using semi-continuous way, based on the optimal conditions, the cell population could obtain over 6.5×10^10 cfu/mL, as almost 10 times as before.

关 键 词：微生物 酒类酒球菌SD-2a 高密度培养 生长曲线 

分 类 号：TS261.11[轻工技术与工程—发酵工程]