高效液相色谱法测定酿酒酵母中S-腺苷-L-蛋氨酸  

The Determination of S-adenosyl-L-methionine in Saccharomyces Ceverisiae by High Performance Liquid Chromatography

作　　者：刘坚[1] 崔海容 郭坚 胡德聪 刘恭源[1] 杨广笑[1] 何光源[1] 

机构地区：[1]华中科技大学HUST-Rres中英基因工程和基因组学联合实验室,武汉430074 [2]湖北省出入境检验检疫局技术中心,武汉430022

出　　处：《分析科学学报》2007年第2期163-165,共3页Journal of Analytical Science

基　　金：国家自然科学基金(No.30370807);国家"973"计划项目(No.2002CB111302)

摘　　要：利用高效液相色谱(HPLC)法对酿酒酵母(Saccharomyces Ceverisiae,SC)发酵样品中S-腺苷-L-蛋氨酸(SAM)的测定方法进行研究。流动相为甲醇∶40 mmoL/LNH4H2PO4-8 mmoL/L 1-庚烷磺酸钠=20∶80(V/V),流速为1.0 mL/min,检测波长为254 nm,用外标法进行定量。方法的相对标准偏差和平均回收率分别为0.82%和98.22%。该方法简便有效,适用于SAM系列产品生产中的质量控制。The method for determination of S-adenosyl-L-methionine（SAM） in fermentation samples of Saccharomyces Ceverisiae （SC）by high performance liquid chromatogram （HPLC） was studied. Methanol ：water （40 mmoL/L NH4H2PO4,8 mmoL/L sodium-1-heptanesulfonate ） at 20：80 （V/V） was used as mobile phase. Flow rate was 1.0 mL/min with detection wavelength of 254 nm. The content of SAM was determined by external standard method. The relative standard deviation and average recovery were 0.82% and 98.22%, respectively. The experimental results show that the method is simple,effective, and can be used for quality control of functional products of SAM.

关 键 词：酿酒酵母 S-腺苷-L-蛋氨酸 HPLC 

分 类 号：O657.72[理学—分析化学]