n-6/n-3多不饱和脂肪酸对乳腺癌大鼠乳腺癌组织CYP17和CYP19表达的影响  

Effect of n-6/n-3 PUFA dietary of different constituent ratio on expressions of CYP17,CYP19 mRNA in rat breast tissues of MNU-induced breast cancer

作　　者：王静[1] 糜漫天[1] 韦娜[1] 王斌[1] 

机构地区：[1]第三军医大学军事预防医学院营养与食品卫生学教研室重庆市营养与食品安全重点实验室,重庆400038

出　　处：《第三军医大学学报》2007年第8期674-677,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30500408);达能营养研究与实验基金(2004)~~

摘　　要：目的探讨不同n-6/n-3多不饱和脂肪酸(PUFA)膳食构成比对MNU诱导的乳腺癌大鼠血清雌二醇水平及乳腺组织CYP17、CYP19 mRNA表达的影响。方法雌性SD大鼠65只随机分为5组,即n-6 PUFA组、10∶1 n-6/n-3组、5∶1 n-6/n-3组、1∶1 n-6/n-3组和正常对照组,前4组以50 mg/kg MNU单次腹腔注射诱导大鼠乳腺肿瘤发生,正常对照组注射等体积无菌生理盐水。给药后立即分组喂养不同饲料,并在8周和18周时经阴道涂片证实间情期,股动脉取血液并剖取乳腺组织,采用放射免疫法检测血清雌二醇水平,利用RT-PCR技术检测乳腺组织中CYP17、CYP19 mRNA表达状况。结果4组乳腺癌大鼠血清雌二醇水平及乳腺组织中CYP17、CYP19的表达较正常对照组均有所升高,其中n-6组最高,其余各组随n-6/n-3值减小而下降,1∶1 n-6/n-3组显著低于n-6组。结论不同n-6/n-3 PUFA膳食构成比对MNU诱导的乳腺癌大鼠血清雌二醇水平及乳腺组织CYP17、CYP19 mRNA的表达具有不同影响,1∶1 n-6/n-3 PUFA膳食构成比能有效抑制乳腺癌大鼠血清雌二醇水平及乳腺组织CYP17、CYP19 mRNA表达的升高。Objective To observe the effect of n-6/n-3 polyunsaturated fatty acid dietary of different constituent ratio on the serum level of estradiol and expressions of CYP17, CYP19 in MNU-induced rat breast cancer tissues. Methods Totally 65 female SD rats were randomly divided into 5 groups： n-6 PUFA, 10：1 n-6/n- 3 PUFA, 5：1 n-6/n-3 PUFA, 1：1 n-6/n-3 PUFA and normal control. Except control group receiving physiological saline, all rats were given a single intraperitoneal injection of MNU （50 mg/kg） and switched to different diets containing fatty acids mentioned above. After 8 and 18 weeks, the serum level of estradiol and expressions of CYP17, CYP19 in the breast tissues of different groups were determined by RIA and RT-PCR. Results The serum level of estradiol and expressions of CYP17, CYP19 in rat breast cancer tissues were higher than normal control, highest in n-6 group and gradually decreased with the ratio of n-6/n-3 decreasing. Conclusion n-6/n-3 PUFA dietary of different constituent ratio can diversely influence the serum level of estradiol and expressions of CYP17, CYP19 in MNU-induced rat breast cancer tissues, n-6/n-3 PUFA dietary with constituent ratio of 1：1 can significantly inhibit the increased serum level of estradiol and increased expressions of CYP17, CYP19 in breast cancer tissues.

关 键 词：N-6/N-3多不饱和脂肪酸 乳腺癌大鼠 血清雌二醇 CYP17 CYP19 

分 类 号：R151.2[医药卫生—营养与食品卫生学] R394.3[医药卫生—公共卫生与预防医学]