莲原花青素对小鼠黑色素瘤的体内抑制作用及机制研究  

Inhibitory Effects of Proanthocyaidins from Lotus Seedpod(LSPC)on Mice B16 Melanoma

作　　者：段玉清[1] 张海晖[1] 吴佳[2] 谢笔钧[2] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]华中农业大学食品科技学院,湖北武汉430070

出　　处：《食品科学》2007年第4期332-335,共4页Food Science

基　　金：江苏省自然科学基金项目(05KJB550011);江苏大学人才基金项目(05JDG013);教育部博士点基金项目(20040504013)

摘　　要：目的:研究莲房原花青素(LSPC)对黑色素瘤B16的抑制作用及其机制。方法:给小鼠连续灌胃不同剂量的LSPC 13d后,于第14d处死小鼠,计算抑瘤率并进行形态学观察,通过免疫组织化学观察瘤细胞内HMB45和S-100蛋白表达,流式细胞术检测细胞凋亡。结果:一定剂量的LSPC能抑制黑色素瘤B16细胞生长增殖,且以120mg/kg·bw LSPC组最显著(p＜0．01),抑瘤率达55．3%,并有亚二倍凋亡峰。结论:LSPC对黑色素瘤B16细胞有抑制作用,其机制是诱导凋亡。Objective： To investigate the inhibitory effects of proanthocyaidins from lotus seedpod （LSPC） on mice B 16 melanoma. Methods： The mice were fed with different dosage LSPC continuely for 13d, on the 14th day, all mice were killed. and the inhibition rate of B 16 melanoma was calculated. The changes of apoptosis cells in morphology were observed, and the expression of HMB45 and S-100 protein in B 16 melanoma were tested by immune histochemistry method. Results： LSPC can inhibit the proliferation of B 16 melanoma. The dosage of 120mg/kgobw shows the most significantly difference in comparison with the control（p 〈 0.01）. The inhibition rate of B 16 melanoma reaches 55.3%, and the cell apoptosis analysis presents a sub-peak. Conclusions： LSPC has the inhibitory effects on the B 16 melanoma and the mechanism is to induce the apoptosis.

关 键 词：莲房 原花青素 黑色素瘤B1 6 抑制作用 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]