天冬氨酸转氨酶反应过程及其在L苯丙氨酸制备中的应用  

RESEARCH ON ASPARTATE TRANSAMINASE REACTION AND PREPARATION OF L PHENYLALANINE IN BSTR AND PFR REACTORS

作　　者：潘光亮[1] 李越人[1] 马伟军 何学军 欧阳平凯[1] 

机构地区：[1]南京化工大学生物工程与科学系

出　　处：《南京化工大学学报》1996年第A01期21-24,共4页Journal of Nanjing University of Chemical Technology(Natural Science Edition)

基　　金："八五"科技攻关项目

摘　　要：利用具有高活性天冬氨酸转氨酶（ＥＣ２６１１）的大肠杆菌（ＥＳｃｈｅｒｉｃｈｉａｃｏｌｉ）变异株细胞，对转氨反应的过程进行了研究。测得游离大肠杆菌细胞及其固定化细胞为酶源参与的转氨反应的表观米氏常数分别为０３９０ｍｏｌ／Ｌ和０４６７ｍｏｌ／Ｌ。同时，发现辅助因子５′磷酸吡哆醛溶液预处理细胞对酶具有明显的激活作用。比较了不同的金属离子对酶的激活和抑制作用。此外，利用固定化细胞。The results of research on the process of enzymic transamination by a mutant strain of Escherichia coli with high aspartate aminotransferase activity were reported. The apparent Michaeli′s Menten constants of free cell catalyzed and immobilized cell catalyzed transmination were determined as 0.39 mol/L and 0.467mol/L respectively. The enzymic activity can be activated by the pretreatment of pyridoxal 5′ phosphate solution and metal ion solution of Mn 2+ ,while it is obviously inhibited by metal ion Ca . The results of preparation of L phenylalanine in BSTR and PFR reactor were also reported.

关 键 词：天冬氨酸 激活 苯丙氨酸 转氨酶 氨基酸 

分 类 号：TQ464.7[化学工程—制药化工]