表皮生长因子受体在血管紧张素Ⅱ促血管平滑肌细胞增殖中的作用  

The Role of Epidermal Growth Factor Receptor in Proliferation of Vascular Smooth Muscle Cells Activated by Angiotensin Ⅱ

作　　者：尹小龙[1] 朱艳霞[2] 姚雨凡 雷芸[1] 

机构地区：[1]昆明市延安医院心内科,云南省昆明市650051 [2]昆明医学院附属第一医院心内科,云南省昆明市650032

出　　处：《中国动脉硬化杂志》2006年第9期783-785,共3页Chinese Journal of Arteriosclerosis

摘　　要：目的探讨表皮生长因子受体在血管紧张素Ⅱ促大鼠血管平滑肌细胞增殖效应中的作用。方法用反义表皮生长因子受体寡核苷酸脂质体复合物转染SD大鼠血管平滑肌细胞,用逆转录聚合酶链反应、Western-Blot-ing分别检测转染后表皮生长因子受体mRNA及蛋白的表达情况,用氚标胸腺嘧啶脱氧核苷掺入实验检测血管平滑肌细胞的增殖情况。结果反义组大鼠血管平滑肌细胞表皮生长因子受体mRNA表达(0.18±0.03)较正义组(0.61±0.11)及对照组(0.66±0.09)明显减少(P<0.05),反义组大鼠血管平滑肌细胞表皮生长因子受体蛋白的表达(43.1±8.4)较正义组(92.6±10.5)及对照组(100.7±11.3)明显减少(P<0.05);反义组细胞的氚标胸腺嘧啶脱氧核苷掺入率(1055.1±95.7)较正义组(1882.4±129.7)及对照组(2013.3±121.3)明显降低(P<0.05)。结论表皮生长因子受体在血管紧张素Ⅱ促血管平滑肌细胞增殖中起重要作用。Aim To investigate the role of epidermal growth factor receptor （EGFR） in proliferation of vascular smooth muscle cells （VSMC） activated by angiotensin Ⅱ （AngⅡ）. Methods The cultured VSMC of Sprague-Dawley rat were transfered with antisense sequences of EGFR. EGFR mRNA and protein in VSMC was detected by reverse transcription polymerase chain reaction （RT-PCR） and western blot analysis, the proliferation of VSMC was determined by 3H-deoxythymidine （3H-TdR） incorporation. Results The expression of EGFR mRNA in VSMC transfected with antisense sequences of ECFR were significantly lower than transfected with sense sequances of EGFR and control group （0.18±0.03, 0.61±0. 11, and 0.66±0.09 respectively, P〈0.05）.The expleasion of EGFR protein in VSMC tramfected with anfisense sequences of EGFR were significantly knver than transfected with seine sequances of EGFR and control group （43.1 ±8.4, 92.6± 10.5, and 100.7± 11.3 respectively, P〈0.05）.The H-TdR incorporation of VSMC transfered with antisense sequences of EGFR were significantly lower than that transfered with sense sequances of ECFR and control group （1 055.1±95.7, 1882.4±129.7, and 2013.3±121.3 respectively, P〈0.05）.Conclusion EGFR play an important role in proliferation d VSMC d rat induced by anglotensin Ⅱ.

关 键 词：病理学与病理生理学 表皮生长因子受体 血管紧张素Ⅱ 血管平滑肌细胞 细胞增殖 氚标胸腺嘧啶脱氧核苷掺入实验 

分 类 号：R363[医药卫生—病理学]