鳕碎肉酶解物清除羟自由基作用研究  

Scavenging Effect of the Hydrolysates from Fish Offal of Pollock on Hydroxyl Free Radicals

作　　者：胡文婷[1] 张凯 孙谧[3] 

机构地区：[1]海南大学海洋学院,海南海口570228 [2]海南亚洲制药有限公司,海南海口570201 [3]中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《水产科学》2007年第4期207-209,共3页Fisheries Science

基　　金：国家"863"计划资助项目(2003AA625070);海南大学科研基金资助项目(kyjj0618)

摘　　要：通过测定鳕碎肉酶解物对Fenton体系产生的羟自由基的清除效果,从海洋蛋白酶YS-894、海洋蛋白酶YS-80、胃蛋白酶和木瓜蛋白酶中,筛选出海洋蛋白酶YS-894作为酶解鳕碎肉制备具有较高清除羟自由基活性酶解物的水解酶;用正交试验L9(34)对该酶的水解条件进行优化,并确定了水解的最佳肉水比。最终工艺条件为40℃、酶解45 m in、pH 9.0、酶解质量分数0.25%、底物∶水=1∶3的条件下进行水解,水解物对羟自由基的清除效果较好,清除率为68.88%。According to the scavenging activity produced by Fenton reaction, marine protease YS-894, whose hydrolysates had higher scavenging activity, had been chosen to be the good hydrolytic enzymes from the four applied enzymes such as marine protease YS-894, marine protease YS-80, pepsin and papain. Furthermore, the optimal hydrolysis of marine protease YS-894 was determined by orthogonal design L9 （ 3^4 ）. Finally, the optimal enzyme/ substances ratio of marine protease YS-894 was studied. The results showed that the optimal enzymatic hydrolysis conditions were temperature 40 ℃ ,time duration 45 min, pH 9.0, enzyme content 0.25% and enzyme/substances ratio 1 ： 3, and that the highest scavenging activity was 68.88% .

关 键 词：鳕 下脚料 酶解 活性肽 羟自由基 

分 类 号：Q514.3[生物学—生物化学]