用于分离小分子肽的凝胶电泳的改进研究  

Improvement on Gel Electrophoresis Method for Separating Small Molecule Polypeptide

作　　者：江龙法[1] 钱志刚[2] 杨海麟[1] 陈中[2] 王武[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214036 [2]淮海工学院海洋学院,江苏连云港222005

出　　处：《化学世界》2007年第4期200-202,共3页Chemical World

摘　　要：为了有效分离小分子肽,对SDS-PAGE方法进行改进,调整分离胶分子组成,并在分离胶中加入36%的尿素或13%甘油。实验结果证明:当凝胶的交联度为7.5-10,浓度大于15%,并加入36%的尿素或13%甘油,可以有效分离相对分子质量大于4.1 kDa的小肽。To separate the small peptides, the SDS-PAGE method was improved. When the cross-linking degree of bisacrylamide with acrylamide was approximately 5 - 10, the spacer gel concentration was greater than 15 %, and 36 % of urea or 13% of glycerol was added in the spacer gel, peptides with ultra-low the relative molecular mass of 4.1 kDa were separated effectively.

关 键 词：SDS-聚丙烯酰胺凝胶电泳 小分子肽 分离 

分 类 号：O648.17[理学—物理化学]