海洋苯酚降解菌Candida sp.P5的分离鉴定及其降解特性  

Screening of a Marine Phenol-degrading Yeast Candida sp.P5 and its Biodegradation Characteristics

作　　者：胡忠[1] 吴奕瑞[1] 徐艳[1] 黄通旺[1] 郑天凌[2] 

机构地区：[1]汕头大学生物学系,广东汕头515063 [2]厦门大学生命科学学院,福建厦门361005

出　　处：《应用与环境生物学报》2007年第2期243-247,共5页Chinese Journal of Applied and Environmental Biology

基　　金：霍英东教育基金会基金(No.942002);国家自然科学基金(No.40206015);广东省科技计划项目(2005B33201008)资助~~

摘　　要：从海洋沉积物中分离、筛选到一株能以苯酚作为唯一碳源和能源的酵母菌P5.根据菌落特征、菌体形态、生理生化特性和18S rDNA序列分析,确定菌株P5为假丝酵母菌属(Candida sp.).该菌株最适宜生长和降解苯酚的条件为:温度25℃,pH6.0～7.0,摇床转速100r/min,需氧;菌株P5能在较高浓度的苯酚条件下生长,在72h内可以降解95%以上的苯酚.对苯酚代谢途径和相关酶的研究发现,菌株P5主要在邻苯二酚1,2-双加氧酶作用下通过邻位途径进行苯酚代谢.Strain P5, capable of degrading phenol, was isolated from marine sediment and could use phenol as the sole carbon source for growth. It was identified as Candida sp. by its morphologic and physiological characteristics, especially by the analysis of 18S rDNA sequences. Its optimal conditions for growth and degradation were 25 ℃, pH 6.0 N7.0, 100 r/min of shaking rate and oxygen required. Strain P5 could grow at a relative higher concentration of phenol and degrade over 95% of phenol within 72 h. The metabolism of phenol was catalyzed by catechol 1,2-dioxygenase of Candida sp. P5 through the meta pathway. Fig 7, Tab 2, Ref 24

关 键 词：苯酚 生物降解 假丝酵母菌P5 分离 鉴定 海洋污染 

分 类 号：X172[环境科学与工程—环境科学]