阿托伐他汀钙对大鼠脑缺血再灌后ICAM-1表达的影响  

Effects of Atorvastatin on Expression of ICAM-1 After Cerebral Ischemia/reperfusion Injury in Rats

作　　者：谷文萍[1] 肖慧[1] 黄蕾[1] 宋小明[1] 刘福中[1] 李小军[1] 李伟[1] 

机构地区：[1]中南大学湘雅医院神经内科,湖南长沙410008

出　　处：《实用预防医学》2007年第2期275-277,共3页Practical Preventive Medicine

基　　金：教外司留学回国基金(教外司留[2006]331号);湖南省自然科学基金(02JJY3028);湖南省卫生厅课题(B2005023)

摘　　要：目的探讨阿托伐他汀钙对大鼠脑缺血再灌后脑组织中ICAM-1蛋白表达的影响。方法采用大脑中动脉线栓法制备局灶性脑缺血再灌模型,参考Longa的5分制法在动物麻醉清醒后进行评分,应用免疫组化法检测ICAM-1蛋白表达,运用HE染色观察多形核白细胞(PMNLs)浸润情况。结果大鼠脑缺血再灌注后缺血脑组织中ICAM-1蛋白阳性血管数表达增加(P<0.01),24 h达高峰;予他汀干预治疗后能减少缺血脑组织中ICAM-1蛋白阳性血管数表达(P<0.05),抑制PMNLs渗出(P<0.05),减少神经功能缺损评分(P<0.05)。结论阿托伐他汀钙能抑制大鼠脑缺血再灌后脑组织中ICAM-1蛋白表达,减少炎症细胞渗出,减轻缺血再灌损伤。Objective To investigate the impact of atorvastatin on the expression of ICAM- 1 in the rat＇s brain tissue after ischemia/ reperfusion（I/R） injury. Methods Rats were subjected to cerebral I/ R injury through the occlusion of the middle cerebral artery. Neurological deficits were determined according to the Longa＇s score. The expression of ICAM - 1 was detected by immuno- histochemical technique. The effusion of PMNLs in brain tissue was evaluated by HE staining. Results The expression of ICAM- 1 in the ischemic territory increased after cerebral ischemia（P〈 0.01 ）, and peaked at 24h after ischemia. Atorvastatin treatment decreased the expression of ICAM- 1 （P〈 0.05）, inhibited the effusion of PMNLs （P〈 0.05）, and improved the Longa＇ s score （ P 〈 0.05） . Conclusion Atorvastatin - calcium protects the brain tissue against ischemia reperfusion injury through inhibiting the expression of ICAM- 1 after focal cerebral ischemia reperfusion in rats.

关 键 词：阿托伐他汀钙 脑缺血再灌 ICAM-1 炎症反应 

分 类 号：R743[医药卫生—神经病学与精神病学]