^(131)I-McAbJHⅢFab′片段在荷瘤裸鼠体内的定位显像  

Radioimmunoimaging of 131 I Labeled Monoclonal Antibody JHⅢFab′ in Nude Mice Bearing Human Hepatocellular Carcinoma

作　　者：苏金华[1,2] 颜江华[1,2] 俞浩 李东辉[1,2] 

机构地区：[1]厦门大学抗癌研究中心 [2]厦门市第一医院同位素室

出　　处：《细胞与分子免疫学杂志》1997年第1期39-42,27,共5页Chinese Journal of Cellular and Molecular Immunology

摘　　要：用蛋白酶切技术制备ＭｃＡｂＪＨⅢＦａｂ′片段，回收率约１０％。片段活性相当于完整ＭｃＡｂ的１１０２稀释浓度。用氯胺Ｔ法标记ＭｃＡｂＪＨⅢＦａｂ′，对荷人肝细胞癌ＢＥＬ－７４０２裸鼠体内的放射免疫显像进行了研究。腹腔注射１３１Ｉ－ＭｃＡｂＪＨⅢＦａｂ′后２４～９６ｈ，肿瘤部位有放射性浓聚，以４８～７２ｈ影像最为清晰；而注射１３１Ｉ－ＭｃＡｂＪＨⅢ肿瘤部位也有积聚现象，但本底消除更慢。１３１Ｉ－ＭｃＡｂＪＨⅢＦａｂ′注射后７２ｈ，１３种器官的肿瘤组织与非瘤组织的放射性比值均大于３，肿瘤的定位指数为２．５６。McAb JHⅢFab′ fragment was prepared with pepsin digestion It′s receiving percentage was about 10% Its titer was 1∶10 2 of McAb JHⅢ McAb JHⅢFab′ fragment was radiolabeled with 131 I by Ch T method Its distribution and radioimmunoimaging in the nude mice bearing human hepatoma BEL 7402 were studied Between 24-96 h after intraperitoneal administration, the image of the concentrating radioacitivity in tumor was obtained by the γ camera and it was the clearest during 48-72 h; whereas the injection of 131 I McAb JHⅢ into the nude mice resulted in an equal radioactivity in tumor, but it′s background was more slowly cleared. At 72 h after the injection of 131 I McAb JHⅢFab′, the radioactivity ratioes of tumor/normal tissue(T/NT) for 13 kinds of organ/tissue were all over 3, and the localization index of tumor was 2 56 These results showed that McAb JHⅢFab′ may be useful in the localization and targeting treatment of human hepatocellular carcinoma

关 键 词：单克隆抗体 酶解作用 肝细胞癌 裸鼠 放射免疫显像 

分 类 号：R735.704[医药卫生—肿瘤]