小麦蛋白质含量分子标记辅助选择的效果分析  

Effect of Marker-assisted Selection on Protein Content in Wheat

作　　者：陈喜文[1] 陈德富[1] 李永君[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《华北农学报》2007年第2期39-42,共4页Acta Agriculturae Boreali-Sinica

基　　金：天津市应用基础研究计划面上项目(043612011);南开大学2004年科技创新基金项目

摘　　要：对小麦回交后代的蛋白质含量进行了分子标记辅助选择研究。先对2个与蛋白质含量相关的Xgwm570和E41M62-168标记进行验证,表明其与蛋白质含量密切相关。再通过表型选择、分子标记辅助选择(MASBC2)和不加选择(RNDBC2)产生的BC2中,蛋白质含量的平均值和群体中蛋白质含量高于16.5%的植株比例在PHEBC2和MASBC2之间没有显著差异,但均显著高于RNDBC2。对群体中的植株按两分子标记的类型分为A(2个位点)、B(1个位点)和C(无)3大类,蛋白质含量的平均值和植株中蛋白质含量高于16.5%的比例从高到低的排列顺序为:A类植株>B类植株>C类植株。由此看来,利用分子标记辅助选择来培育高蛋白质含量小麦是十分有效的方法。Marker-assisted selection for protein content was conducted in backcross population in wheat. Experiments were designed first to confirm the linkage of molecular markers Xgwm570, E41M62-168 with protein content. Comparison with backcross population developed through phenotypic selection （PHEBC2）, by marker-assisted selection （MAS- BC2） and by random mating where no selection has been applied （RNDBC2）, there was no significant difference between means of phenotypic and marker-assisted selection （16.2 % ） for protein content and the percentage of plants which have protein content higher than 16.5%, but significantly higher than RNDBC2 population. When the backcross-derived plants were divided into A （two loci）, B （one locus） and C （null） according to their molecular marker types, their protein content values and the percentage of plants which have protein content higher than 16.5 % ranked from high to low is type A 〉 type B 〉 type C. From these we could conclude that marker-assisted selection is an efficient method to breed protein content in wheat.

关 键 词：小麦 蛋白质含量 分子标记辅助选择 数量性状位点(QTL) 

分 类 号：S511[农业科学—作物学]