瘦素对大鼠主动脉平滑肌细胞迁移和增殖的影响  

The Effects of Leptin on the Migration and Proliferation in Vascular Smooth Muscle Cell

作　　者：王丽[1] 唐澜[1] 赵勇[1] 黄芬[1] 王华兵[1] 王洪杰[1] 曾和松[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院心内科,湖北省武汉市430030

出　　处：《中国动脉硬化杂志》2006年第11期937-939,共3页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金资助项目(30470713)

摘　　要：目的探讨瘦素对大鼠主动脉平滑肌细胞迁移和增殖的影响,以阐明瘦素在动脉粥样硬化发生发展机制中的作用。方法体外培养大鼠主动脉平滑肌细胞至第4~6代,分别用不同浓度的瘦素（0、20、40、80、100、200μg/L）孵育24 h后：模拟Boyden实验,检测瘦素对平滑肌细胞趋化迁移的影响;MTT法检测瘦素对平滑肌细胞生长的影响;流式细胞仪检测瘦素对平滑肌细胞增殖指数的影响。结果瘦素对平滑肌细胞的生长有促进作用,这种促增殖效应呈剂量依赖性,在瘦素浓度为100μg/L时达最大效应（OD值为0.193±0.010）。瘦素对平滑肌细胞增殖指数的影响与此相同。瘦素有明显的促进平滑肌细胞趋化迁移作用,而且随着瘦素浓度的增加,迁移的细胞数目明显增多。结论瘦素可以促进平滑肌细胞的迁移和增殖,提示瘦素可能有促进动脉粥样硬化的作用。Aim To investigate the effects of leptin on the migration and proliferation of vascular smooth muscle cell （VSMC） invitro. Methods The smooth muscle cells at passage of 4 ~ 6 were incubated with leptin in different concentrarices （0, 20, 40, 80, 100, 200μg/L） for 24 hours, then modified Boyden＇s experiment was used to measure the effect of leptin on migration; MTT was used to measure the dosage-effect curve of leptin on the growth of VSMC; and flow cytometry （ FC, M） was used to measure the effect of leptin on the cell cyclin. Results The growth of VSMC was promoted with the concentration of leptin, and reached the maximum （OD：0. 193 ± 0.010） at 100 μg/L, so did the effect of leptin on the proliferation index of VSMC. Leptin can promote the migration of VSMC, and the number of migrated ceil was promoted with the increase of leptin concentration. Conclusion Leptin can promote the migration and proliferation of VSMC in vitro, which explicits the effects of leptin in the at herosclerosis.

关 键 词：病理学与病理生理学 瘦素 平滑肌细胞 迁移 增殖 动脉粥样硬化 大鼠 

分 类 号：R363[医药卫生—病理学]