光皮木瓜总三萜化合物提取和含量测定  

Extraction and Determination of Total Triterpenes from Chaenomeles sinensis (Thouin) Koehne

作　　者：洪艳平[1] 尹忠平[1] 上官新晨[1] 杨武英[1] 蒋艳[1] 沈勇根[1] 

机构地区：[1]江西农业大学食品科学与工程学院植物资源开发与利用研究室,江西南昌330045

出　　处：《江西农业大学学报》2007年第2期225-229,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江西省科技厅农业攻关项目(200305001)

摘　　要：通过分光光度法定量,研究了光皮木瓜中总三萜化合物的乙醇浸提工艺。在单因素实验基础上采用L9(34)正交试验设计,考察了浸提时间、浸提温度、料液比等因素对光皮木瓜总三萜化合物提取率的影响。结果表明,在无水乙醇体系中浸提温度为80℃,浸提时间为3 h,料液比为1∶16时提取效果最好,总三萜化合物提取率为2.83%。The extraction technology for total triterpenes from Chaenomeles sinensis （Thouin） Koehne with ethanol as extracting liquid, and the total triterpenes was determined through ultraviolet spectrophotometry. The effect factors of temperature, treatment time and mass/volume ratio of solvent were studied through orthogonal tests L^9 （3^4） on the basis of single factor test. The results showed that the extraction rate of total triterpenes was 2.83% in the optimal extracting conditions as follows： 16 times of amount of anhydrous alcohol, 80 ℃ of extraction temperature and 3 hours of extraction time.

关 键 词：光皮木瓜 总三萜化合物 浸提 分光光度法 

分 类 号：Q94-331[生物学—植物学]