半边莲生物碱抑制肾性高血压大鼠内皮素1 mRNA和蛋白表达  

Lobelia Chinensis Lour Alkaloids Inhibit Expression of Endothelin-1 mRNA and Protein in Renal Hypertensive Rats

作　　者：张晓玲[1] 薛冰[1] 李莉[1] 陈融[1] 任冬梅[2] 李瑞峰[1] 胡维诚[1] 

机构地区：[1]山东大学医学院 [2]山东大学药学院,山东省济南市250012

出　　处：《中国动脉硬化杂志》2007年第1期11-14,共4页Chinese Journal of Arteriosclerosis

基　　金：教育部高等学校博士学科点专项科研基金(39730210);山东省自然科学基金(Q2004C03)

摘　　要：目的观察半边莲生物碱对肾性高血压大鼠内皮素1 mRNA表达、蛋白合成和释放的影响。方法采用两肾一夹高血压大鼠模型,随机分为高血压组、半边莲组、卡托普利组和假手术组。用原位杂交技术观察大鼠外周血白细胞内皮素1 mRNA的表达,应用免疫组织化学染色计数大鼠主动脉内皮细胞铺片内皮素阳性细胞率(反映内皮素1蛋白的合成),用放射免疫技术测定大鼠血浆内皮素的含量。结果与假手术组比较,高血压组外周血白细胞内皮素1mRNA表达增强(34.64%±8.39%比9.34%±4.47%,P<0.05),动脉内皮细胞合成内皮素增多(7.42%±0.24%比1.58%±0.24%,P<0.05),血浆内皮素水平显著升高(221±24ng/L比138±19ng/L,P<0.05)。应用半边莲生物碱8周后,与高血压组比较,内皮素1 mRNA表达(20.38%±11.31%比34.64%±8.39%,P<0.05)、内皮素合成(3.53%±0.21%比7.42%±0.24%,P<0.05)和血浆内皮素水平(191±21ng/L比221±24ng/L,P<0.05)均受到显著抑制。结论肾性高血压大鼠伴有内皮素表达增强,半边莲生物碱能抑制内皮素基因的转录、蛋白合成及翻译,对防治肾性高血压所致的血管病变具有一定作用。Aim To investigate the effects of the Lobelia Chinensis Lour Alkaloids（LCLA） on endothelin-1（ET-1） mRNA expression and endothelin synthesis and release in renal hypertensive rats （RHR）. Methods The glodblatt renovascular hypertension model was induced in rats by two-kidney one clip method. After 4 weeks the rats were divided into 3 groups, including the hypertensive group, the captopril group and the LCLA group, while the control group was given sham-operation. Using in situ hybridization to observe ET-1 mRNA expression in peripheral blood leucocytes. Using immunohistochemical technique to count positive rate of ET in ＂en face＂ preparations of arterial endothelium to reflect the synthesis of ET. Using radioimmunoassay to examine the level of ET in serum, Results Compared with the sham operation group, the positive rate of ET-1 mRNA in peripheral blood leucocytes （ 34.64% ± 8.39 % vs 9.34 % ± 4.47 %, P 〈 0.05 ）, the positive rate of ET in arterial endothelium （7.42% ± 0.24% vs 1.58% ± 0.24%, P 〈 0.05）and the plasma coneentration of ET（221 ± 24 ng/L vs 138 ± 19 ng/L, P 〈0.05） were increased significantly in hypertensive rats. After treating with LCLA for 8 w, compared with the hypertensive group, ET-1 mRNA expression （20.38% ± 11.31% vs 34.64% ± 8.39%, P 〈0.05）, endothelin （ET） synthesis （3.53% ± 0.21% vs 7.42% ±0.24%, P〈0.05） and release（191 ±21 ng/L vs 221 ±24 ng/L, P〈0.05） were significantly inhibited. Conclusions The expression of ET was increased in RHR. The LCLA can inhibit the expression of ET in the transcriptional and translational level which may be effective to the preventing and treatment of the renal hypertension.

关 键 词：病理学与病理生理学 半边莲生物碱 内皮素1 肾性高血压 原位杂交 免疫组织化学染色 放射免疫技术 

分 类 号：R363[医药卫生—病理学]