Beneficial effect of an antibody against interleukin-2 receptor (daclizumab) in an experimental model of hepatocyte xenotransplantation  

作　　者：Dimitrios Papagoras Apostolos Papalois Alexandra Tsaroucha Dimitrios Lytras John Kyriazanos Nikoletta Giannakou Prodromos Laftsidis Constantine Simopoulos 

机构地区：[1]2~(nd) Department of Surgery, Medical School, "Democritus" University of Thrace, Alexandroupolis,Greece [2]2~(nd) Department of Surgery, Medical School, "Democritus" University of Thrace, Alexandroupolis,Greece,Experimental-Research Department, Elpen Pharmaceuticals, Athens, Greece [3]Department of Pathology, "Aghia Olga" General Hospital, Athens,Greece

出　　处：《World Journal of Gastroenterology》2007年第9期1435-1437,共3页世界胃肠病学杂志（英文版）

摘　　要：AIM: To investigate the use of Daclizumab (Dmab) as an immunosuppressive agent in an experimental model of hepatocyte xenotransplantation (XenoTx) in rats with fulminant hepatic failure (FHF). METHODS: Two white male New Zealand rabbits were used as donors and 68 Wistar rats as recipients. FHF was induced by intravenous application of dimethylnitrosamine (DMNA). The isolated hepatocytes of the rabbits were xenotransplanted into the spleen of the rats 24 h after FHF induction. Group A (n = 13): no treatment; Group B (n = 14): FHF and XenoTx; Group C (n = 14): FHF and XenoTx and cyclosporin (CsA); Group D (n = 14): FHF and XenoTx and Dmab; Group E (n = 13): FHF and XenoTx and CsA and Dmab. The rats were followed for 15 d. RESULTS: Statistical analysis showed better survival among groups D (92.86%) and E (76.92%) compared to group A (all rats died after 72 h), group B (28.57%) or group C (71.43%), although the differences were not statistically significant. Biochemical evaluation of the liver enzymes and histology confirmed satisfactory function and engraftment, respectively. CONCLUSION: This experimental model has shown the safe, effective and beneficial use of Dmab in a xenotransplantation model of rabbit hepatocytes in rats.AIM： To investigate the use of Daclizumab （Dmab） as an immunosuppressive agent in an experimental model of hepatocyte xenotransplantation （XenoTx） in rats with fulminant hepatic failure （FHF）. METHODS： Two white male New Zealand rabbits were used as donors and 68 Wistar rats as recipients. FHF was induced by intravenous application of dimethylnitrosamine （DMNA）. The isolated hepatocytes of the rabbits were xenotransplanted into the spleen of the rats 24 h after FHF induction. Group A （n = 13）： no treatment; Group B （n = 14）： FHF and XenoTx; Group C （n = 14）： FHF and XenoTx and cyclosporin （CsA）; Group D （n = 14）： FHF and XenoTx and Dmab; Group E （n = 13）： FHF and XenoTx and CsA and Dmab. The rats were followed for 15 d. RESULTS： Statistical analysis showed better survival among groups D （92.86%） and E （76.92%） compared to group A （all rats died after 72 h）, group B （28.57%） or group C （71.43%）, although the differences were not statistically significant. Biochemical evaluation of the liver enzymes and histology confirmed satisfactory function and engraftment, respectively. CONCLUSION： This experimental model has shown the safe, effective and beneficial use of Dmab in a xenotransplantation model of rabbit hepatocytes in rats.

关 键 词：Transplantation HEPATOCYTES DACLIZUMAB CYCLOSPORIN 

分 类 号：R657.3[医药卫生—外科学]