鸭舌草中抗氧化活性物质的分离与鉴定  

Isolation and identification of an antioxidant from Monochoria vaginalis

作　　者：周勇军[1] 徐效华[2] 乔凤云[1] 张建萍[1] 余柳青[1] 

机构地区：[1]中国水稻研究所水稻生物学国家重点实验室,杭州310006 [2]南开大学元素有机化学国家重点实验室,天津300071

出　　处：《应用生态学报》2007年第3期509-513,共5页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金资助项目(30571231).

摘　　要：采用溶剂萃取法将鸭舌草75%乙醇提取物浸膏分成4个不同组分,并用碘量法测定其抗氧化活性.结果表明,高极性组分正丁醇相具有最强的抗氧化活性,与天然抗氧化剂茶多酚和化学合成抗氧化剂BHT的抗氧化活性相当,显著高于对照.利用柱层析技术对具有高抗氧化活性组分正丁醇相进一步分离纯化,并确定为豆甾醇葡萄糖甙.以BHT为对照对羟自由基进行清除试验,结果表明,与对照抗氧化剂BHT相比,它们对羟自由基具有更高的清除率.With solvent extraction, a total of four fractions were separated from 75% alcohol extract of Monochoria vaginalis, and their antioxidative activities were measured by iodine method. The resuits showed that among the fractions, n-butanol fraction exhibited the highest antioxidative activity, which was not only significantly higher than CK （water）, but also equivalent to the natural antioxidant tea polyphenols and synthetic antioxidant butylated hydroxyl toluene （BHT）. A compound was isolated from the n-butanol fraction by using column chromatography, and identified as stigmasterol 3-O-β-D-glucopyranoside. Its antioxidative activity estimated by the determination of the percentage of scavenged free radicals indicated that this compound exhibited a higher activity than BHT in scavenging free radicals.

关 键 词：鸭舌草 抗氧化活性 分离 鉴定 食品安全 

分 类 号：Q946[生物学—植物学] S451[农业科学—植物保护]