反义寡核苷酸抑制大鼠损伤视神经Nogo-A mRNA的表达  

Effects of antisense oligodeoxynucleotides on Nogo-A mRNA expression in injured optic nerves

作　　者：袁容娣[1] 叶剑[1] 彭锡嘉[1] 刘少章[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所眼科,中国重庆市400042

出　　处：《国际眼科杂志》2007年第2期354-356,共3页International Eye Science

基　　金：中国国家自然科学基金资助项目(No.30270458);军队十五医学科研基金资助项目(No.01MA175)~~

摘　　要：目的:观察反义寡核苷酸对损伤视神经Nogo-A的影响。方法:实验分为对照组、随机序列组、和2,5,10μmol/L3种Nogo-A反义寡核苷酸浓度组。大鼠视神经钳夹伤后,采用逆转录-聚合酶链反应法(RT-PCR),半定量分析反义寡核苷酸对损伤视神经Nogo-A mRNA表达量的变化。结果:反义寡核苷酸均显著降低Nogo-A mRNA的表达(P<0.01),随机序列对Nogo-A mRNA的表达无影响(P>0.05)。结论:反义寡核苷酸对视神经损伤后Nogo-A mRNA表达有抑制作用。AIM： To observe the effects of antisense oligodeoxynudeotides （ASODN） on Nogo-A mlRNA expression in injured optic nerves. METHODS： A total of 30 rats were divided into five groups： control group, random sequence group and test groups （2μmol/L, 51μmol/L and 101μmol/L Nogo-A antisense oligodeoxynudeotides）, reverse transcriptase-polymerase chain reaction （RT-PCR） method was used in the detection of the levels of Nogo-A mRNA in injured optic nerves. RESULTS： The result of RT-PCR showed that Nogo-A antisense oligodeoxynudeotides could significantly inhibit the expresson of Nogo-A mRNA （P〈0.01）, random sequence antisense oligedeoxynudeotides had no noticeable effect on Nogo-A mRNA. CONCLUSION： Nogo-A antisense oligodeoxynudeotides can inhibit the expresson of Nogo-A mRNA in injured opticnerves.

关 键 词：反义寡核苷酸 NOGO-A 视神经 创伤 RT-PCR 

分 类 号：R779.1[医药卫生—眼科]