siRNA抑制survivin的表达诱导人卵巢癌SKOV-3细胞凋亡的研究  

Induction of human ovarian carcinoma cell line SKVO-3 apoptosis by siRNA through targeting to survivin

作　　者：王建军[1] 刘彧[1] 王宏卫[2] 廖小玲[2] 朱诗应[2] 李怀芳[1] 

机构地区：[1]同济大学附属同济医院妇产科,上海200065 [2]第二军医大学微生物学教研室,上海200433

出　　处：《同济大学学报（医学版）》2007年第2期14-18,共5页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金资助项目(30500606)

摘　　要：目的筛选能有效抑制survivin基因表达的小干扰RNA(siRNA),探讨survivin基因抑制对人卵巢癌SKOV-3细胞凋亡的影响。方法设计3段survivin siRNA靶位点,用PCR扩增siRNA表达框,与survivin-绿色荧光蛋白融合基因表达质粒共转染293T细胞,再构建有效siRNA的表达载体,转染卵巢癌SKOV-3细胞,分析sur-vivin基因的表达及细胞凋亡情况。结果筛选出了1段能高效抑制survivin表达的siRNA,能显著下调SKOV-3细胞内的survivin表达,并导致SKOV-3细胞凋亡。结论利用RNA干扰能有效抑制survivin基因表达并诱导人卵巢癌SKOV-3细胞凋亡,为进一步的靶向survivin分子的体内抗肿瘤研究奠定了基础。Objective To screen small interference RNAs that could inhibit survivin expression and to explore the effect of RNA interference of survivin on apoptosis of ovarian carcinoma carcinoma. Methods Three siRNA target sequences for survivin were designed and the siRNA expression cassettes were prepared by PCR. The siRNA expression cassettes were transfected into 293T cells along with survivin and green fluorescent protein fusion gene expression plasmid. The expression vector of an effective siRNA was constructed and transfected into ovarian carcinoma SKVO-3 cells, and the expression of survivin and apoptosis were assayed. Results A candidate siRNA that down-regulated the expression of survivin in SKVO-3 cells was selected, which could inhibit expression of survivin in SKVO-3 cells and lead to apoptosis actively. Conclusion siRNA can suppress expression of survivin and induce SKVO-3 cells apoptosis effectively, which might lay a foundation for further research on the treatment of malignant tumor through targeting to survivin.

关 键 词：SURVIVIN RNA干扰 卵巢肿癌 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤]