共患破坏性行为障碍的儿童注意缺陷多动障碍与5-HT2C基因多态的相关性研究  

Association between serotonin 2C gene polymorphisms and attention deficit hyperactivity disorder in children with or without comorbidity of disruptive behavior disorder

作　　者：李君[1] 王玉凤[1] 周儒伦[1] 杨莉[1] 张浩波[1] 汪冰[1] 

机构地区：[1]北京大学精神卫生研究所儿童研究室,100083

出　　处：《中华儿科杂志》2007年第5期374-377,共4页Chinese Journal of Pediatrics

基　　金：卫生部临床学科重点项目(2004-468);北京市科技计划项目(Y0204003040831);科技部攻关项目(Y0204003040831)

摘　　要：目的探讨共患和不共患破坏性行为障碍(DBD)的儿童注意缺陷多动障碍(ADHD)与5-HT2C 受体基因(HTR2C)C-759T 和 G-697C 两种多态之间的关联。方法就237个共患 DBD的 ADHD 核心家系和251个不共患 DBD 的 ADHD 核心家系的 C-759T 和 G-697C 两种多态进行检测,并对各等位基因和单体型进行传递不平衡检验(TDT)。结果在共患 DBD 的家系中未发现任何等位基因和单体型的传递不平衡现象。在不共患 DBD 的家系中,-759C(X^2=4.25,P=0.04)优先传递,-697G(X^2=3.21,P=0.07)有优先传递的趋势,就单体型而言,-759C/-697G 优先传递(X^2=4.31,P=0.04)。结论 ADHD 共患 DBD 与否在 HTR2C 基因 C-759T 和 G-697C 两种多态水平上有明显的遗传差别。HTR2C 基因与不共患 DBD 的 ADHD 相关联。Objective Disruptive behavior disorder （DBD） is one of the main comorbidity of attention deficit hyperactivity disorder （ADHD）. Previous studies showed significantly different serotonin function between ADHD children with and without the comorbidity of DBD. Therefore, it is needed to compare these two groups in terms of serotonin receptor gene poiymorphisms, which may provide further evidence for the previous studies. The current study aimed to investigate the relationship between two serotonin receptor 2C （ HTR2C ） gene polymorphisms, that are C-759T and G-697C polymorphisms, and ADHD with or without concomitant DBD. Method Blood samples were taken from 237 trios with probands of ADHD with DBD comorbidity and 251 trios with probands of ADHD without comorbidity of DBD. All the subjects were from the ADHD clinic of Peking University Sixth Hospital. DNA was extracted and PCR was performed to amplify the fragments containing both C-759T and G-697C polymorphisms. AciI was used to detect different alleles of the two polymorphisms. Both allele-based and haplotype-based TDT analyses were used to test the association of the two polymorphisms of HTR2C gene and ADHD with or without comorbidity of DBD. Results The haplotypes -759C （X^2 = 4. 25,P = 0. 04）, -697G（ X^2 = 3.21,P = 0. 07）, as well as - 759C/-697G were over- transmitted （ X^2 = 4. 31, P = 0. 04 ） to the probands of ADHD without DBD. No biased transmission of any allele and haplotype were found in families with probands of ADHD with DBD. Conclusion ADHD with or without the comorbidity DBD was different at the level of HTR2C gene polymorphisms of C-759T and G-697C. HTR2C is related to ADHD without DBD, while not related to ADHD with DBD. The results suggested that the two groups may have different genetic background, at least in HTR2C.

关 键 词：注意力缺陷障碍伴多动 受体 血清素 5-HT2C 连锁不平衡 单元型 多态 现象(遗传学) 

分 类 号：R749.94[医药卫生—神经病学与精神病学]