高效液相色谱法测定大豆胚轴中各类皂甙的含量  

Determination of soyasaponins from soybean hypocotyl by HPLC

作　　者：全吉淑[1] 尹学哲[1] 工藤重光 

机构地区：[1]延边大学医学院,延吉133000 [2]日本东北食效科学研究所,青森0300842

出　　处：《食品科技》2007年第4期172-174,共3页Food Science and Technology

基　　金：国家自然科学基金项目(30360113)

摘　　要：建立了大豆皂甙的高效液相色谱-差示折光(HPLC-dRI)检测方法。色谱条件为:ODS-AM-303色谱柱(YMC,4.6mm×250mm,5μm),柱温40℃,含0.1%三氟乙酸的乙腈-水(40∶60)为流动相,流速1mL/min。A1、A2、Ba、Bb、Bd、Be、αg和βg分别在1.39～6.96μg、1.67～8.34μg、2.24～11.2μg、2.35～11.8μg、1.58～7.92μg、2.01～10.1μg、1.38～6.88μg和1.62～8.12μg范围内线性关系良好,平均回收率分别为91.3%、92.6%、92.5%、93.8%、94.1%、95.8%、93.4%和94.2%,RSD为4.63%、3.48%、3.22%、3.18%、4.01%、3.53%、4.07%和4.28%。方法准确度高,重现性好,适用于大豆皂甙样品中各类皂甙的测定。High-performance liquid chromatographic-differential refractive index detective （HPLC-dRI） method was established to determine soyasaponins. The chromatographic conditions are as fellows： ODS-AM-303 column （YMC, 4.6mm×250mm, 5μm） with the temperature of 40℃, acetonitrile-water （40：60） containing 0.1% trifluo roacetic acid as mobile phase with the flow rate of 1mL/min. The method was proved to be linear in ranges of 1.39-6.96μg, 1.67-8.34μg, 2.24-11.2μg, 2.35-11.8μg, 1.58-7.92μg, 2.01-10.1μg, 1.38-6.88μg and 1.62-8.12μg for A1, A2, Ba, Bb, Bd, Be, ag and 15g, respectively; the recoveries were 91.3%, 92.6%, 92.5%, 93.8%, 94.1%, 95.8%, 93.4% and 94.2% with RSD of 4.63%, 3.48%, 3.22%, 3.18%, 4.01%, 3.53%, 4.07% and 4.28% for A1 and A2, Ba, Bb, Bd, Be, αg and βg, respectively. The method is accurate and reproducible, suitable for the determination of sovasaponins.

关 键 词：大豆胚轴 大豆皂甙 高效液相色谱法 

分 类 号：TS207.3[轻工技术与工程—食品科学]