Expressed sequence tags analysis of a liver tissue cDNA library from a highly inbred minipig line  

作　　者：CHEN You-nan TAN Wei-dong LU Yan-rong QIN Sheng-fang LI Sheng-fu ZENG Yang-zhi BU Hong LI You-ping CHENG Jing-qiu 

机构地区：[1]Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China [2]Laboratory of Banna Minipig Inbred Line, Yunnan Agricultural University, Kunming 650000, China

出　　处：《Chinese Medical Journal》2007年第9期739-742,共4页中华医学杂志（英文版）

基　　金：This study was supported by grants from the National Natural Science Foundation of China(No.30270679);National Basic Research Program of China(No.2003CB515504);National Basic Research Program of China(No.2004CCA01800).

摘　　要：Background Porcine liver performing efficient physiological functions in the human body is prerequisite for successful liver xenotransplantation. However, the protein differences between pig and human remain largely unexplored. Therefore we investigated the liver expression profile of a highly inbred minipig line. Methods A cDNA library was constructed from liver tissue of an inbred Banna minipig. Two hundred randomly selected clones were sequenced then analysed by BLAST programme. Results Alignments of the sequences showed 44% encoded previously known porcine genes. Among the 56% unknown genes, sequences of 72 clones had high similarities with known genes of other species and the similarities to human were mostly above 0.80. The other 40 clones showing no similarity to genes in National Centre for Biotechnology Information are newly discovered, expressed sequence tags specific to liver of inbred Banna minipig. Twenty-two of the 200 clones had full length encoding regions, 38 complete 5＇ terminal sequences and 140 complete 3＇ terminal sequences.Conclusion These newly discovered expression sequences may be an important resource for research involving physiological characteristics and medical usage of inbred pigs and contribute to matching studies in xenotransplantation.Background Porcine liver performing efficient physiological functions in the human body is prerequisite for successful liver xenotransplantation. However, the protein differences between pig and human remain largely unexplored. Therefore we investigated the liver expression profile of a highly inbred minipig line. Methods A cDNA library was constructed from liver tissue of an inbred Banna minipig. Two hundred randomly selected clones were sequenced then analysed by BLAST programme. Results Alignments of the sequences showed 44% encoded previously known porcine genes. Among the 56% unknown genes, sequences of 72 clones had high similarities with known genes of other species and the similarities to human were mostly above 0.80. The other 40 clones showing no similarity to genes in National Centre for Biotechnology Information are newly discovered, expressed sequence tags specific to liver of inbred Banna minipig. Twenty-two of the 200 clones had full length encoding regions, 38 complete 5＇ terminal sequences and 140 complete 3＇ terminal sequences.Conclusion These newly discovered expression sequences may be an important resource for research involving physiological characteristics and medical usage of inbred pigs and contribute to matching studies in xenotransplantation.

关 键 词：Banna minipig inbred line cDNA library LIVER expressed sequence tags XENOTRANSPLANTATION 

分 类 号：Q78[生物学—分子生物学] R394.8[医药卫生—医学遗传学]