中国人胃癌KAI1基因表达及遗传不稳定性  

Expression and Genetic Instability of KAI1 Gene in Chinese with Gastric Cancer

作　　者：杨月琴[1] 陈学荣[2] 郑枫芸[1] 张仙土 李继承[1] 

机构地区：[1]浙江大学细胞生物学研究所,杭州310031 [2]浙江省台州市第一人民医院,黄岩318020

出　　处：《细胞生物学杂志》2007年第2期246-250,共5页Chinese Journal of Cell Biology

摘　　要：采用Envision免疫组织化学,Leica-Qwin计算机图像分析,石蜡包埋组织抽提DNA,PCR-单链构象多态性(SSCP)和常规银染等方法,对56例石蜡包埋胃癌标本及其相应的正常组织,进行KAI1蛋白表达水平的研究和D1S1344、D11S1326位点微卫星不稳定(MSI)、杂合性缺失(LOH)的检测,为揭示KAI1基因作用机制和肿瘤转移机制提供实验依据。实验中,胃癌KAI1蛋白阳性检出率为55.4%(31/56):随着癌组织浸润程度的进展,其阳性率呈降低趋势(P<0.01);在无淋巴结转移的肿瘤组织KAI1蛋白表达率为83.9%,显著高于淋巴结转移肿瘤组织的20.0%;在肿瘤结节转移(tumor node metastasis,TNM)Ⅰ+Ⅱ期,KAI1蛋白阳性率为82.8%,明显高于TNMⅢ+Ⅳ期的25.9%(P<0.01)。56例胃癌D11S1326、D11S1344位点的SSCP分析中,均未出现MSI或LOH。实验结果提示,KAI1蛋白表达与胃癌组织浸润、淋巴结转移及恶性进展密切相关。在胃癌的发生发展中,KAI1基因未见遗传不稳定性改变。The aim of the study was to study the expression of KAI1 protein and the genetic instability of KAI1 gene, and their relationship with clinical pathological behaviors in Chinese with gastric cancer, which will illustrate the evaluation of tumor metastasis and the prognosis in gastric carcinoma, and provide experimental basis for the mechanism of KAI1 gene. Techniques such as Envision immunohistochemistry and Leica-Qwin computer imaging techniques were used to assess the expression of KAI1 protein. DNA extraction from formalin-fixed paraffinembedded tissues, polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP）, ordinary silver stain were used to study microsatellite instability （MSI） and loss of heterozygosity （LOH） of locus D11S1344 and D 11S 1326. In our experiment, the expression rate of KAI1 protein was 55.4% （31/56）. With the infiltration of tumor, the frequency of KAI1 protein appeared decrease tendency （P〈0.01）. Moreover, the frequency in lymph node metastasis cases was （83.9%） significantly higher than those without （20.0%, P〈0.01）. Stage TNM Ⅰ＋Ⅱ （82.8%） also exhibited higher frequency of KAI1 protein than stage TNM Ⅲ＋Ⅳ （25.9%, P〈0.01）. However, none of 56 gastric cancers showed LOH or MSI at locus D 11S 1344 and D11S 1326. The results indicated that the expression of KAI1 protein has great relationship with the infiltration of tumor, the lymph node metastasis and the prognosis of gastric carcinoma. There is rare LOH or MSI in KAI1 gene in gastric carcinoma.

关 键 词：KAI1基因 胃癌 免疫组化 微卫星不稳定 杂合性缺失 

分 类 号：R735.2[医药卫生—肿瘤]