新疆沙冬青抗冻蛋白的提取分离及其热滞活性测定  

Extraction,Separation and Measuration of the Thermal Hysteresis Activity of Antifreeze Proteins from Ammopiptanthus nanus (Leguminosae)

作　　者：尉姗姗[1] 尹林克[1] 牟书勇[1] 鲁春芳[1] 赵峰侠[1] 

机构地区：[1]中国科学院新疆生态与地理研究所,新疆乌鲁木齐830011

出　　处：《云南植物研究》2007年第2期251-255,共5页Acta Botanica Yunnanica

基　　金：重大基础研究前期研究专项(2004CCA03400);新疆维吾尔自治区自然科学基金项目(200421106);新疆生物资源基因工程重点实验室开放课题(XJDX0201-2005-09)

摘　　要：抗冻蛋白被发现于低温环境下生存的生物体中,它能够吸附在冰晶表面并改变冰晶的生长形态,对于增加植物的抗低温胁迫能力有重要作用。用纤维素DE-52离子柱层析提取分离出了冬季新疆沙冬青(Ammopiptanthus nanus)叶片中的抗冻蛋白(AFPs)。差示扫描量热法(DSC)测定结果表明,当蛋白浓度为20mg/ml时,抗冻蛋白的热滞活性(THA)为0·46℃。经SDS-PAGE电泳分析,此抗冻蛋白的分子量为119·24kDa。Antifreeze proteins （AFPs）, because of their ability of adsorbing onto the surface of ice crystals and modifying their growth, have been found in many different organisms that are exposed to chilling and freezing temperatures. It is very important to increase the resisting and coercing ability at low temperature. DE-52 ion-exchange cellulose was used to extract and separate the antifreeze proteins from Ammopiptanthus nanus leaves. Differential scanning calorimetry （DSC） was used to measure the thermal hysteresis activity （THA）. The results reveal that when the concentration of the protein is 20 mg/ml, the THA of the AFPs is 0.46℃. Its molecular weight is about 119.24 kDa by sodium dodecyl sulfate polyaerylam ide gel electmphoresis （SDS-PAGE）.

关 键 词：新疆沙冬青 抗冻蛋白 差示扫描量热法 热滞活性 

分 类 号：Q946[生物学—植物学]