非小细胞肺癌中MTAP与p16纯合缺失与突变分析  

Study on the mutations and Homozygous deletions of MTAP and p16 in NSCLC

作　　者：李邦印[1] 张开泰[2] 霍艳英[2] 胡迎春[2] 徐勤枝[2] 周平坤[2] 吴德昌[2] 

机构地区：[1]解放军总医院第二附属医院,北京100091 [2]军事医学科学院辐射与放射医学研究所,北京100850

出　　处：《临床肺科杂志》2007年第6期547-548,555,共3页Journal of Clinical Pulmonary Medicine

基　　金：国家重点基础研究发展规划973项目(批准号G1998051207)基金资助

摘　　要：目的研究非小细胞肺癌中MTAP与p16纯合性缺失与点突变发生频率,探讨MTAP与肺癌的相关性。方法提取44例原发性非小细胞肺癌标本(21例腺癌和23例鳞癌)的基因组DNA;设计MTAP、p16基因外显子及其参照基因MTAP的引物,以基因组DNA为模板,进行PCR扩增,通过SSCP分析基因点突变现象,通过PCR-ELISA分析基因纯合性缺失现象。结果SSCP方法分析发现在44例原发性肺癌标本中,p16和MTAP没有点突变现象;PCR-ELISA分析发现p16基因的纯合性缺失频率为6.8%,MTAP的纯合性缺失频率9.1%,统计学分析表明二基因之间的缺失频率无显著性差异,但二者均与非小细胞肺癌显著相关。结论与p16类似,MTAP纯合性缺失是原发性非小细胞肺癌中发生的重要事件之一,有其肿瘤生物学基础。Objective To investigate frequency of Homozygous deletions of MTAP and pl 6, and to find out the key role of MTAP different from pl 6. Methods Genomic DNA was extracted from 44 non-small cell lung cancers （NSCLC） samples. Primers for MTAP exons and p16 exon 1α were synthesized. SSCP was used to screen mutations of MTAP and p16 gene. Homozygous deletions of MTAP and p16 gene were quantitatively analyzed by PCR-ELISA. Results No Mutations of MTAP and p16 gene were found in all cases by SSCP. By PCR-ELISA, Frequencies of Homozygous deletions of MTAP and pl 6 were 9. 1 percent and 6. 8 percent respectively. Conclusion Homozygous deletions of MTAP may also play a key role in NSCLC development.

关 键 词：MTAP P16 非小细胞肺癌 纯合性缺失 

分 类 号：R734.2[医药卫生—肿瘤]