醇醛脱氢酶基因多态和饮酒习惯与肝癌易感性  

Relationship among ADH2 and ALDH2 genotypes, alcohol drinking and risk of primary hepatocellular carcinoma

作　　者：丁建华[1] 李苏平[1] 吴建中[1] 高长明[1] 周建农[1] 曹海霞[1] 苏平[1] 刘燕婷[1] 周学富[2] 常军[2] 

机构地区：[1]江苏省肿瘤防治研究所流行病室,江苏南京210009 [2]泰兴市疾病控制中心,江苏泰兴225400

出　　处：《中华肿瘤防治杂志》2007年第7期500-504,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：1999年江苏省社会发展基金重点资助项目(BS99026-1);江苏省"333新世纪学术带头人"资金资助项目(2002)

摘　　要：目的研究乙醇脱氢酶2（ADH2）和乙醛脱氢酶2（ALDH2）基因多态及饮酒习惯与肝癌的易感性。方法对208例原发性肝癌和208例对照调查饮酒习惯,采用RCT-RFLR方法检测ADH2和ALDH2基因型。结果1）病例与对照ADH2和ALDH2基因型分布频率差异均无统计学意义。2）携带AL-DH2^1＊2或ALDH2^2＊2基因型且饮酒总量〉3kg年者,发生肝癌危险性是携带AL-DH211基因型不饮酒者的3.30倍（95%CI=1.24～8.83）;而携带ADH2^1＊2或ADH2^2＊2基因型者且饮酒总量〉3kg年与携带ADH211基因型不饮酒者相比,患肝癌危险性无显著增加。3）携带AL-DH2^1＊2或ALDH2^2＊2同时携带ADH2^1＊2或ADH2^2＊2基因型且饮酒总量〉3kg年者,与携带ALDH2^1＊1同时携带ADH2^1＊1基因型且饮酒总量≤3kg年者相比,患肝癌OR值虽有增加但未达显著性（OR=4.26,95%CI=0.63～29.08）。4）HBsAg阳性并携带ALDH2^1＊2或ALDH2^2＊2基因型且饮酒〉3kg年者,与HBsAg阴性并携带ALDH2^1＊1基因型且饮酒≤3kg年者相比,患肝癌危险升高49.71倍（95%CI=5.51～448.96）。结论大量饮酒和肝癌的关联与ALDH2基因有关,而与ADH2基因无关。OBJECTIVE：To investigate the relationship among aldehyde dehydrogenase-2 （ALDH2）, alcohol dehydrogenase-2 （ADH2） genotypes and alcohol drinking for the susceptibility of primary hepatocellular carcinoma （PHC）. METHODS： A case-control study including 208 cases of PHC and 208 controls was carried out in Taixing Blood samples were collected and tested for ALDH2 and ADH2 genotypes by PCR-RFLP method. RESULTS： 1）The frequencies of ALDH2 and ADH2 genotypes were no significantly different between the PHC cases and controls. 2） Compared with those nodrinkers with ALDH2^1＊1 genotypes, drinkers with ALDH^2＊2 or ALDH2^2＊2 genotypes and cumulative amount of alcohol consumption 〉3 （kg ＊ years） were at a significantly higher risk of developing PHC （OR=3.30, 95%CI= 1.24 8.83）. When compared with those with different ADH2 genotypes, no significant difference was found （OR=1.46,95%CI=0.39-5.47）. 3）Drinkers with ALDH^1＊2 or ALDH2^2＊2 genotypes and ADH2^1＊2 or ADH2^2＊2 genotypes and cumulative amount of alcohol consumption 〉 3 （kg ＊ years） were not at a significantly risk of developing PHC （OR = 4.26, 95%CI=0.63-29.08） compared with those no-drinkers with ADH2^1＊1 and ALDH2^1＊1 genotypes. 4）Compared with individuals with ALDH2^1＊1 , negative of HBsAg and cumulative amount of alco hol consumption ≤3 （kg ＊ years）, those with ALDH2^1＊2 or ALDH2^2＊2 , positive of HBsAg and cumulative amount of alcohol consumption 〉3 （kg ＊ years） were at a significantly higher risk of PHC （OR=49.71, 95%CI=5.51-448.96）. CONCLUSIONS.. These resuits reveal that not ADH2 but ALDH2 polymorphisms have a significantly interaction with heavy alcohol consumption in the development of PHC.

关 键 词：病例对照研究 醇脱氢酶/遗传学 多态现象 遗传 肝肿瘤/流行病学 肝肿瘤/遗传学 基因型 疾病遗传易感性 饮酒 

分 类 号：R735.7[医药卫生—肿瘤]