十二烷基苯磺酸钠共振散射光谱法测定木瓜蛋白酶活力  

Resonance Scattering Spectral Assay of Papain Enzymatic Activity with Sodium Dodecyl Benzene Sulfonate

作　　者：黄国霞[1] 蒋治良[1,2] 梁爱惠[3] 邓业成[1] 

机构地区：[1]广西师范大学环境与资源学院 [2]桂林工学院材料与化学工程系,广西桂林541004 [3]桂林工学院材料与化学工程系

出　　处：《光谱学与光谱分析》2007年第5期1003-1005,共3页Spectroscopy and Spectral Analysis

基　　金：广西自然科学基金项目(0575042);广西"新世纪十百千人才工程"计划资助

摘　　要：在pH值6．5的磷酸盐缓冲溶液中，十二烷基苯磺酸钠（SDBS）与酪蛋白（Casein）形成缔合物微粒，在470，360，400，420和520nm产生5个瑞利散射峰。在选定条件下，木瓜蛋白酶（Papain）可水解酪蛋白（Casein），加SDBS可中止酶催化反应并与未反应的酪蛋白底物结合形成缔合物微粒。随着Papain浓度的增大，470nm处的共振散射峰强度降低。Papain的酶活力在0．048-4．8USP·mL^-1范围内与△Ⅰ470nm呈现良好的线性关系。其线性回归方程为△ⅠSDBS=1.972c＋2．31，相关系数分别为r=0．9999，检测限为0．020USP·mL^-1。该法用于嫩肉粉中木瓜蛋白酶活力测定，结果令人满意。In pH 6.5 phosphate buffer solutions, dodecyl benzene sulfonate （SDBS） was combined with casein to form association particles, which exhibited five Rayleigh scattering peaks at 470, 360, 400, 420 and 520 nm, respectively. Under suitable conditions, papain has catalytic effect on the hydrolysis of casein, and SDBS can stop the catalytic reaction and be combined with the excess casein to form association particles. The scattering peak at 470 nm decreased with the activity of papain. The △Ⅰ470nm value was linear with the papain activity in the range of 0. 048-4. 8 USP·mL^-1. Its regress equation is △ⅠSDBS = 1. 972c＋2.31, with a related coefficient of 0. 999 9 and detection limit of 0. 020 USP ·mL^-1. This new assay has been applied to the assay of the papain activity in food additive with satisfactory results.

关 键 词：木瓜蛋白酶 酪蛋白 十二烷基苯磺酸钠 共振散射光谱法 

分 类 号：O657.6[理学—分析化学]