快速检测食品中违禁偶氮染料“对位红”的电化学方法的建立  

Rapid determination of banned azo colourant para-red in food by electro-chemical method

作　　者：刘国艳[1] 柴春彦[1] 葛宇[2] 宋巍巍[1] 

机构地区：[1]上海交通大学农业与生物学院,上海201101 [2]国家食品质量监督检验中心,上海200233

出　　处：《食品工业科技》2007年第5期224-226,共3页Science and Technology of Food Industry

基　　金：上海市科委技术标准专项资助项目(05dz05009)。

摘　　要：以玻碳电极为工作电极,将对位红衍生化后,用电化学方法进行测定。结果表明,衍生化后的“对位红”表现出明显的电化学行为。运用导数循环伏安法测定“对位红”时,在扫描电压0.725V处出现了明显氧化峰,氧化峰电流与“对位红”衍生物浓度呈现良好线性关系,线性回归方程为Y=0.99315+741.26X,相关系数r=0.99238(p<0.0001),检测限达0.014mg/L,RSD为4.5%,准确度为96%,平均回收率为95.63%,检测每个样品所需时间不多于3min。由此可知,本实验建立的电化学方法能够实现快速、灵敏检测“对位红”的要求。To establish a electro-chemical method for rapidly determining the banned azo colourant para-red, a glass carbon electrode （GCE） was used as working electrode, and para-red was derivated. The results showed that derivated para -red molecules presented obvious electrochemical behaviors. There was a good liner relationship between the peak current of derivated para-red molecutes oxidized at the potential of 0.725V and the content of para-red, the regression equation was Y=0.99315＋741.26X,r= 0.99238 （p 〈0.0001）. The detecting limit reached 0.014mg/L,the RSD,the rate of accuracy and recovery were 4.5%, 96%, 95.63%, respectively. The detecting time for one sample wasn＇t more than 3 minutes. It can be concluded that the novel electro-chemical method for para-red analysis is rapid and sensitive.

关 键 词：对住红 电化学方法 检测 

分 类 号：TS207.5[轻工技术与工程—食品科学]