白芍总苷提取和纯化工艺的研究  

Study on Extraction and Purification of Total Glucosides of Paeony from Radix Pueoniae Alba

作　　者：李钐[1] 王亚楠[1] 刘小宇[1] 张学文[1] 丛培江[1] 

机构地区：[1]青岛大学生物系,山东青岛266071

出　　处：《食品科学》2007年第5期173-176,共4页Food Science

摘　　要：采用L9（3^4）的正交试验设计，采用水浸提，以加水量、提取时间、提取次数，作为主要考虑因素，确定了白芍总苷的最佳提取工艺为：加入12倍量的水煎煮三次，每次3h。其中提取次数是主要影响因素。在此基础上，采用D101大孔吸附树脂分离纯化，最终白芍中芍药苷转移率可达80．5％。The optimum extracting process of total glucosides of Peaony from Radix Pueoniae Alba by aqueous method was obtained after using L9（34） orthogonal test plan. We selected three elements - water amounts, extraction hours and extraction times to observe their effects on the extraction of total glucosides of Peaony. The orthogonal test results showed that the optimum conditions were as follows＂ the water is 12 times of the Radix Pueoniae Alba, it is decocted 3 times and each time is sustained for 3 hours. The major factor affecting the content of total glucosides is extraction times. On the basis of these experiments, we used D101 macroreticuler resin to purify the extraction and the final content of total glucosides is up to 80.5%.

关 键 词：白芍总苷 芍药苷 提取 纯化 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]