双载体法固定化黑曲霉发酵产壳聚糖酶的研究  

Chitosanase Production by Immobilized Aspergillus niger Cells

作　　者：周桂[1] 何子平[2] 禤金彩[1] 

机构地区：[1]广西民族大学化学与生态工程学院,南宁530006 [2]广西农业科学院,南宁530007

出　　处：《西南大学学报（自然科学版）》2007年第5期104-107,共4页Journal of Southwest University(Natural Science Edition)

基　　金：广西青年基金资助项目(桂科青0481036);广西民族大学重点课程建设与教学改革资助项目

摘　　要：探索玉米芯颗粒吸附、海藻酸钠包埋固定化黑曲霉(Aspergillus niger)孢子的方法以及双载体固定化黑曲霉发酵产壳聚糖酶的条件.实验结果表明,玉米芯颗粒对黑曲霉孢子的吸附容量约为5×107个/克;适宜包埋剂质量分数为1·0%;0·2g玉米芯颗粒经吸附、包埋与增殖预培养成熟的固定化黑曲霉细胞发酵产壳聚糖酶最适pH为5·0,适宜发酵装液量为25mL(250mL三角瓶),28·6℃,132r/min条件下摇床发酵48h产壳聚糖酶活力101·3U/mL.Mycelia of Aspergillus niger were absorbed with corncob granule and embedded with sodium alginate. The Immobilization of Aspergillus niger cells was applied in chitosanase production. The max absorbability of corncob granule to the spores of A.niger was 5 X 107/g. The optimized w（sodium alginate） was 1.0%;The optimized pH for chitosanase production by immobilized A. niger cells absorbed with 0.2 g corncob granules and embedded with sodium alginate was 5.0. After 48 hours＇fermentation at 132 r/min and 28. 6℃,the chitosanase activity produced by the mycelia of A. niger was as high as 101.3 U/mL.

关 键 词：黑曲霉 固定化细胞 玉米芯 海藻酸钠 壳聚糖酶 

分 类 号：S188[农业科学—农业基础科学]