阿托伐他汀钙对实验性脑缺血MMP-9表达变化的影响  

Effects of atorvastatin on the expression of MMP-9 after cerebral ischemia/reperfusion injury in rats

作　　者：刘福中[1] 谷文萍[1] 李伟[1] 肖慧[1] 李晓军[1] 宋小明[1] 王妮妮[1] 杨期东[1] 

机构地区：[1]中南大学湘雅医院神经内科,长沙410008

出　　处：《中国神经精神疾病杂志》2007年第5期276-279,共4页Chinese Journal of Nervous and Mental Diseases

基　　金：湖南省卫生厅课题(编号:B2005023);湖南省自然科学基金(编号:02JJY3028);教外司留学回国基金(编号:教外司留[2006]331号)

摘　　要：目的探讨阿托伐他汀钙对大鼠脑缺血再灌注后脑组织中MMP-9mRNA及蛋白表达的影响。方法采用大脑中动脉线栓法制备脑缺血再灌注模型,参考Longa5分制法在动物麻醉清醒后进行评分,应用原位杂交和免疫组化法检测MMP-9mRNA及蛋白表达。结果大鼠脑缺血再灌注后缺血脑组织中MMP-9mRNA和蛋白表达增加(P<0.01),24h达高峰;阿托伐他汀钙干预治疗后能减少缺血脑组织中MMP-9mRNA和蛋白表达(P<0.05);降低神经功能缺损评分(P<0.05)。结论阿托伐他汀钙能抑制大鼠脑缺血再灌注后脑组织中MMP-9mRNA及蛋白表达,减轻缺血再灌损伤。Objective To investigate the impact of the atorvastatin on the expression of MMP-9 in the brain tissue after ischemia/reperfusion（I/R） injury. Methods Rats were subjected to cerebral I/R injury through the occlusion of the middle cerebral artery. Neurological deficits were determined by Longa＇s score. The expression of MMP-9 was detected by immuno-histochemical analysis, and the expression of MMP-9 mRNA was detected by the in situ hybridization method. Restilts The expression of MMP-9 mRNA and protein in the ischemic territory increased after cerebral ischemia（ P 〈 0. 01 ）, peaking at 24h after ischemia. Atorvastatin could decrease the expression of MMP-9 mRNA and protein（ P 〈 0. 05 ） and im- prove the Longa＇s score （ P 〈0. 05） . Conclusions Atorvastatin-calcium protect the（ brain tissue against ischemia reperfusion injury through inhibiting the expression of MMP-9 after focal cerebral ischemia reperfusion in rats.

关 键 词：阿托伐他汀钙 脑缺血再灌注 炎症反应 基质金属白酶9 

分 类 号：R743.3[医药卫生—神经病学与精神病学]