乌骨鸡磷脂侧链脂肪酸的GC-MS分析  

Analysis of Fatty Acid of Phospholipid in Black-bone Silky Fowl by GC-MS

作　　者：王维亚[1] 田颖刚[1] 谢明勇[1] 吴红静[1] 唐永富[1] 

机构地区：[1]南昌大学食品科学教育部重点实验室,江西南昌330047

出　　处：《南昌大学学报（理科版）》2007年第2期150-153,共4页Journal of Nanchang University(Natural Science)

基　　金：教育部长江学者和创新团队发展计划资助项目(IRT0540);江西省农业科技重点攻关资助项目(2005);南昌大学测试基金资助项目(2005029)

摘　　要：用氯仿:甲醇(2:1)超声提取乌骨鸡总磷脂,丙酮脱油精制后,经皂化、甲酯化处理,应用GC-MS联用技术对其侧链脂肪酸组成进行分析,并以面积归一法,计算各脂肪酸的相对百分含量。结果显示,乌骨鸡总磷脂中含有硬脂酸27.46%、花生四烯酸21.39%、油酸18.22%、亚油酸16.67%、棕榈酸12.13%、二十二碳六烯酸(DHA)2.26%、二十碳三烯酸1.71%、棕榈油酸0.16%。不饱和脂肪酸和多不饱和脂肪酸的含量分别为60.41%和42.03%。乌骨鸡磷脂侧链脂肪酸中高比例的多不饱和脂肪酸和花生四烯酸含量是乌骨鸡磷脂的显著特征。Phospholipid was obtained from Black -bone silky fowl with Chloroform -Methanol solvent by ultrasonic sound method. Purified by acetone, phospholipid was saponified and methanolysed. The fatty acids of phospholipid were identified and analyzed by GC - MS and their relative percentage contents were determined by areas normalization method. The results showed that the contents of stearic acid, arachidonic acid, oleic acid, linoleic acid, palmitic acid, DHA , Eicosatrienoic acid and palmitoleic acid were 27.46%, 21.39%, 18.22%, 16.67%, 12. 13%, 2. 26%, 1.71% and 0.16%, respectively. Among them unsaturated fatty acids （UFAs） were about 60.41%, and the polyunsaturated fatty acids （PUFAs） were about 42.03%. The black -bone silky fowl phospholipid was characterized by high contents of PUFAs and AA.

关 键 词：乌骨鸡 磷脂 脂肪酸 气相色谱-质谱 

分 类 号：TS201.2[轻工技术与工程—食品科学]