鼠尾胶原为底物的人鼻腔纤毛上皮细胞培养模式的建立  

Preparation of rat tail collagen and a cell culture model of human nasal epithelial cells

作　　者：宋晓红[1] 张罗[1,2] 韩德民[1,2] 范尔钟[3] 王鸿[3] 王奎吉[1] 李颖[3] 

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科 [2]北京市耳鼻咽喉科研究所,北京100005 [3]北京市耳鼻咽喉科研究所

出　　处：《中国耳鼻咽喉头颈外科》2007年第2期107-111,共5页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金资助项目(30572026);北京市科技新星资助项目(2003-B-15)联合资助

摘　　要：目的建立以鼠尾胶原为贴附底物的人鼻腔纤毛上皮细胞的体外培养模式,为人鼻腔黏液纤毛运输系统的研究提供科学有效的方法。方法制备鼠尾胶原并铺片,以鼠尾胶原为贴附底物组织块培养法培养人鼻腔纤毛上皮细胞,培养7天时进行HE染色及扫描电镜和透射电镜观察细胞形态和结构,应用高速摄像技术测量纤毛摆动频率。结果鼠尾胶原要平坦均匀,平均厚度约为1mm;HE染色可见上皮细胞呈单层向周围爬开;扫描电镜下见纤毛上皮细胞呈不规则多角形,纤毛周围可见微绒毛;透射电镜下可见纤毛上皮细胞间为紧密连接;同一细胞任意两点纤毛摆动频率是相同的;同一来源体外培养的钩突和下鼻甲的纤毛摆动频率是相同的。结论以鼠尾胶原为贴附底物人鼻腔纤毛上皮细胞的体外培养模式的成功建立,为今后研究鼻内用药对纤毛清除功能的影响提供了良好的方法和途径。OBJECTIVE To establish a cell culture of human nasal epithelial cells on glass cover slides coated with rat tail collagen and explore a method to research human mucociliary system. METHODS The rat tail collagen and collagen coated cover slides were prepared for explant culture of human nasal epithelial cells. RESULTS The collagen on cover slides must be thin and fiat. It was about lmm thick. The cultured epithelial cells were monolayer and polygonal cells. There was tight junction between the cells. Ciliary beat frequency of different cilia was equal on the same cell, and it was also equal of on cells from uncinate process and inferior turbinate. CONCLUSION This successful cell culture model of human nasal epithelail cell could be helpful to research the physiology and function of human nasal mucociliary system.

关 键 词：人类 鼻腔 上皮细胞 细胞培养技术 鼠尾胶原 

分 类 号：R-332[医药卫生]