健胃愈疡颗粒干预下大鼠胃溃疡黏膜乳癌相关肽和血小板活化因子的表达及与胃黏膜疏水性的相关性研究  

Association between expressions of PS2 and platelet activating factor and hydrophobicity of gastric ulcer mucosa in rats following treatment with Jianwei Yuyang granule

作　　者：黄国栋[1] 李家邦[1] 黄媛华[2] 黄道富[2] 肖美珍[2] 唐丽君[3] 张曙[3] 李倩[4] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南省长沙市410008 [2]湖南中医学院附属衡阳医院胃肠科,湖南省衡阳市421001 [3]湖南省人民医院肝胆外科,湖南省长沙市410002 [4]湖南中医学院附属第一医院消化科,湖南省长沙市411001

出　　处：《中国组织工程研究与临床康复》2007年第21期4169-4173,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:观察对胃溃疡复发有较好疗效的健胃愈疡颗粒对溃疡黏膜乳腺癌相关肽和血小板活化因子表达的影响,分析其可能的作用机制。方法:实验于2005-07/2006-07在湘雅医院中心实验室完成。SD大鼠110只,雌雄各半,随机抽签法分为5组,即正常对照组、假手术组、雷尼替丁组、健胃愈疡组,各20只;模型组30只。以Okabe改良法复制大鼠实验性胃溃疡,假手术组仅以生理盐水代替乙酸注入玻管内。造模后24h,雷尼替丁组、健胃愈疡组大鼠分别灌服盐酸雷尼替丁和健胃愈疡颗粒(药物组成为:柴胡、党参、白芍、延胡索、白芨、珍珠层粉、青黛、甘草,湖南湘雅制药有限公司生产)药液10mL/kg,分别相当于2.70,1.62g/kg,1次/d。假手术组、模型组灌服蒸馏水10mL/kg。10d后各组中随机取出10只大鼠剖腹取胃(处死前大鼠禁食24h),90d时将模型组20只大鼠再分为模型复发组和模型非复发组,各10只;除正常对照组、假手术组、模型非复发组大鼠腹腔内注射生理盐水外,其余各组大鼠腹腔内注射白细胞介素1,1μg/kg;在注射48h,大鼠禁食24h后,剖腹取胃。观察其对胃溃疡大鼠胃黏膜氨基己糖及磷脂含量、溃疡指数和胃黏膜血流的影响,并用RT-PCR观察乳癌相关肽乳癌相关肽和血小板活化因子表达的变化。结果:实验动物110只,全部进入结果分析。①模型组10,92d胃黏膜血流均低于正常对照组(P<0.01);健胃愈疡组同期胃黏膜血流均高于模型组(P<0.01)。②健胃愈疡组和雷尼替丁组10d溃疡指数均低于模型组(P<0.01,P<0.05);模型复发组、健胃愈疡组和雷尼替丁组92d溃疡指数均高于模型组(P<0.01);健胃愈疡组10,92d溃疡指数及复发率均低于雷尼替丁组(P<0.05,P<0.01)。③模型组10,92d氨基己糖和磷脂含量均低于正常对照组(P<0.01)。健胃愈疡组10,92d氨基己糖和磷脂含量均高于模型组和雷尼替丁组(P<0.01)。溃疡指数与氨基已糖、磷AIM： To observe the influences of Jianwei Yuyang （JWYY） granule, which has good clinical curative effect on preventing the recurrence of gastric ulcer, on expressions of PS2 and platelet activating factor （PAF）, and analyze its potential mechanism. METHODS： The experiment was performed at the Central Laboratory, Xiangya Hospital from July 2005 to July 2006. 110 SD rats, male and female （half and half） were selected and divided into 5 groups, namely normal control group, sham operation group, ranitidine group, JWYY group with 20 in each group, and model group with 30 rats. Gastric ulcer rat models were established by modified Okabe method. Sham operation group only received saline instead of acetic acid by glass-tube. 24 hours after establishing models, ranitidine group and JWYY group received 10 mL/kg ranitidine and JWYY granule （composed of bupleurum root, radix codonopsitis, paeoniae radix, corydalis, common bletilla tuber, nacreous layer powder, indigo naturalis, licorice root, and produced by Hunan Xiangya Pharmacy Co., Ltd.） physic liquor by gastric perfusion, which was equal to 2.70 and 1.62 g/kg, respectively, once a day. Sham operation group and model group received 10 mL/kg distilled water by gastric perfusion. 10 days later, 10 rats of each group were cut the belly open to remove stomach （fasting for 24 hours before killing）. At day 90 20 rats in model group were divided into occurrence model group and non-occurrence model group, 10 in each group. Normal control group, sham operation group and model group received saline by intraperitoneal injection, besides, other groups received 1 μg/kg intedeukin 1 by the same manner. After injection for 48 hours and fasting for 24 hours, rats were cut the belly open to remove stomach. Influences of JWYY granule on contents of aminohexose and phospholipids, ulcer index and blood flow of gastric mucosa in rats with gastric ulcer were observed. RT-PCR was used to observe the changes of the PS2 and PAF expressions. RESULTS： Totally 110 rats we

关 键 词：消化性溃疡/中药疗法 复发 胃粘膜/代谢 磷脂类 疏水性 血小板活化因子 

分 类 号：R573[医药卫生—消化系统]