啤酒与乙醇对γ射线诱发小鼠骨髓细胞染色体畸变的影响  

Effects of Beer and Ethanol on γ Ray-induced Chromosome Aberration in Mouse Bone Marrow Cells

作　　者：吕兑财[1] 赵亚丽[1] 王根良[1] 梁宏[1] 国耀宇[1] 

机构地区：[1]中国航天员科研训练中心,北京100094

出　　处：《航天医学与医学工程》2007年第3期161-164,共4页Space Medicine & Medical Engineering

基　　金：航天医学工程预先研究项目资助(101101306)

摘　　要：目的观察啤酒及乙醇对不同剂量γ射线照射小鼠所诱发的骨髓细胞染色体畸变的影响。方法雄性健康BALB/c小鼠,随机分成对照组、啤酒组和乙醇稀释液组,分别用1ml灭菌生理盐水、啤酒及乙醇稀释液灌胃。30min后给予不同剂量γ射线1次全身照射。照射后24h颈椎离断法处死动物(处死前4h按4μg/g体重腹腔注射秋水仙胺),立即取双侧股骨,进行骨髓细胞常规染色体制片、染色。每一剂量点选取5个个体进行染色体畸变观察。每只动物镜检分析100个细胞分裂相。结果啤酒组及乙醇稀释液组染色体畸变率在照射剂量达0.5Gy以上时明显降低(P<0.05或P<0.01),其中啤酒组的下降比乙醇稀释液组更明显。结论啤酒中除乙醇以外的其他成份(如酚类化合物等)也发挥了降低染色体畸变的作用。ObjectiveTo observe effects of beer and ethanol on chromosome aberrations of mouse bone marrow cells induced by various doses of γ ray irradiation. Methods Healthy male BALB/c mice were arranged into control group,beer group and diluted ethanol group. One ml of saline, beer（ ethanol content, 4.5 v/v） or diluted ethanol （4.5 v/v） were administered orally to the three groups respectively. Thirty minutes later, the animals were given whole-body irradiation with various doses of γ ray. The animals were sacrificed by cervical dislocation 24 h after irradiation. （4 μg/g colchicine was administered to the experimental animals intraperiteonally 4 h before killing）. According to routine methods ,chromosome slides were made with bone marrow from both femurs. Five mice were used for each dose and 100 metaphases for each animal were observed under microscope. Results When irradiation dose was up to 0, 5 Gy, chromosome aberrations induced by irradiation in both beer and ethanol groups decreased obviously（P 〈0.05 or P 〈0.01 ） ,and more significent effect was found in beer group. Conclusion Beside ethanol,other antioxidant substances,such as phenolic compounds,may account for beer＇s activity of reducing chromosome aberrations induced bv irradiation.

关 键 词：啤酒 乙醇 辐射防护 染色体畸变 

分 类 号：R852.7[医药卫生—航空、航天与航海医学]