DNA倍体分析系统对宫颈癌普查的价值  

Cervical cancer screening by using DNA imaging cytometry

作　　者：姚倩瑜[1] 杨琳[1] 

机构地区：[1]东莞市计划生育服务中心检验室,广东省东莞523079

出　　处：《中国基层医药》2007年第5期796-798,共3页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的 探讨用全自动DNA倍体分析系统进行宫颈癌普查的应用价值。方法 对4411名宫颈癌普查的妇女用宫颈刷取材，进行液基薄层制片，分别进行巴氏染色和DNA染色。巴氏染色行常规细胞学检查，用全自动DNA倍体分析系统对DNA染色片进行自动扫描诊断。结果 参加普查妇女中，常规细胞学检查发现异常384例（8．71％），全自动DNA倍体分析系统检查发现异常114例（2．58％）。其中有68例妇女行宫颈组织学活检，以病理诊断结果为标准，全自动DNA倍体分析系统发现3个以上〉5倍体细胞为标准筛查CINⅡ以上宫颈病变的敏感性为78．57％，而常规细胞学方法则为54．76％。结论 用全自动DNA倍体分析系统进行宫颈癌普查能明显提高阳性检出率。Objective To explore the value of using fully automated DNA imaging cytometry in cervical cancer screening. Methods A total of 4411 women were involved in this study. Cervical samples were taken by a cervix brush. Monolayer of cells were deposited onto microscope slides by liquid-based sampling preparation. Two slides were prepared from each case： one slide was stained for conventional cytology examination, while the other slide was stained by Feulgen method for determination of amount of DNA using a fully automated DNA imaging cytometry. Results 384 abnormal cases（ 8.71% ） were found out by conventional cytology exmaination. 114 cases（ 2.58 % ） were found out by DNA image cytometer. A total of 68 women were followed by colposcopy examination where biopsies were taken. The sensitivity of 78.57% were calculated for automated DNA imaging and sensitivity of 54.76% was for conventional cytology. Conclusion The screening for high grade cervical neoplastic lesions and cervical cancer by fully automated DNA imaging could have higher sensitivity.

关 键 词：DNA指纹法 宫颈肿瘤 普查 细胞遗传学分析 

分 类 号：R737[医药卫生—肿瘤]