低能N+离子·紫外线和^(60)Co-γ射线对黑曲霉产木聚糖酶的诱变效应  

Effects of Low Energy N+ Ion,UV and ^(60)Co-γ on Xylanase Produced by Aspergillus niger

作　　者：李市场[1] 古绍斌[1] 姚建铭[2] 余增亮[2] 

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471003 [2]中国科学院离子束生物工程重点实验室,安徽合肥230031

出　　处：《安徽农业科学》2007年第17期5080-5081,5116,共3页Journal of Anhui Agricultural Sciences

基　　金：国家"十五"攻关项目资助(2001BA302B-04)

摘　　要：以木聚糖酶产生菌黑曲霉为试验菌株,比较了低能N+离子、紫外线和60Co-γ射线对黑曲霉的剂量存活效应和诱变效果。结果发现:N+离子注入的存活曲线与紫外线、60Co-γ射线辐照不同,即紫外线和60Co-γ射线照射黑曲霉的剂量存活曲线均是指数曲线,而N+离子注入呈复杂异常损伤的趋势,且3种辐射在诱变效应上有很大的差异。在相同死亡率条件下,紫外线诱变率低,但正变多于负变,而60Co-γ射线诱变率高,负变远远多于正变。适当剂量的N+离子注入表现出诱变率高、正变率高及正变幅度大的特点。Effects of low energy N^＋ ion, UV and ^60Co-γ ray on the survival effect and mutation impact of A spergillus niger were compared, Result showed that the survival curve of N^＋ ion implantation, which showed abnormal damage, were different with those of UV and ^60Co-γ rays, which were both exponential, Comparison of mutation indexes indicated that the N^＋ irradiation was more advantageous than UV and ^60Co-γ rays.

关 键 词：离子注入 黑曲霉 紫外线 60Co-γ射线 异常损伤 木聚糖酶 

分 类 号：Q55[生物学—生物化学]