两株狂犬病病毒强毒株糖蛋白基因的克隆及潜在抗原表位分析  

Cloning and analyzing of potential antigen site of glycoprotein gene of two rabies virus virulent strains

作　　者：崔艳[1] 李忠[1] 王雷[1] 张守峰[1] 扈荣良[1] 

机构地区：[1]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《中国预防兽医学报》2007年第6期479-482,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(30471294)

摘　　要：通过RT-PCR分别获得了狂犬病病毒强毒CVS株、DRV82株糖蛋白基因,进行克隆及测序,并推导出氨基酸序列,与犬用疫苗弱毒株ERA、SRV9、犬源性街毒株CGX及人用疫苗株PG的糖蛋白序列进行比较。结果表明,以上狂犬病病毒毒株间的核苷酸同源性为83.1%～99.2%,氨基酸序列同源性为87.0%～98.5%。经Jameson-Wolf抗原表位优势图分析,CVS株与其他各株相比发现在304位、372位抗原表位优势升高;而DRV82株与其它各株差异不明显。抗原优势变化可能导致狂犬病病毒糖蛋白出现新的潜在抗原位点,为下一步构建不同毒株的狂犬病病毒糖蛋白重组疫苗奠定了基础。Glycoprotein gene of two rabies virus virulent stains, CVS and DRV82, were obtained by RT-PCR, cloned and sequenced. Amino acid sequences of the glycoproteins were compared with these of ERA, SRV9, two live attenuated rabies vaccines for veterinary used, rabies street virus CGX, and rabies vaccine strain PG for human. The results showed that the glcoproteins of rabies virus strains shared 83.1%-99.2 % sequence homology at nucleotide level, and 87.0 %-98.5 % homology at amino acid level. Compared to other virus strains, the CVS strain has an increased hydrophobicity at amino acid 304 and 372 However DRV82 strain has shown no obvious different from other strains. These changes may result in fbnnation of new potentia antigen sites, which could be useful for designing improved recombinant rabies vaccines.

关 键 词：狂犬病病毒 糖蛋白 抗原位点 

分 类 号：S855.3[农业科学—临床兽医学] Q786[农业科学—兽医学]