深层培养中赭曲霉菌球对坎利酮11α羟化反应影响的研究  

Studies on pelleted growth form of Aspergillus ochraceus in submerged cultures as a biocatalyst for canrenone 11αhydroxylation

作　　者：赵玉金[1] 杜连祥[1] 路福平[1] 

机构地区：[1]天津科技大学生物工程学院天津市工业微生物重点实验室,天津300222

出　　处：《工业微生物》2007年第3期20-23,共4页Industrial Microbiology

摘　　要：菌球的松散程度和菌球直径通过调节发酵培养基的初始pH和接种量来实现。实验结果证明直径越小,结构越松散的菌球可以获得高11α羟基坎利酮转化率。7L罐可以形成颗粒小,且松散的菌球。终了转化率92%,且节省了发酵时间。Morphology was manipulated by means of inoculum level and initial pH value. The physiological state of mycelial pellets was estimated according to the growth kintics, pH value, and 11α hydroxy-canrenone conversion ratio. It was found that more efficient biotransformation was performed with small and fluffy pellets. The highest 11α hydroxy-canrenone conversion ratio of 92 % was achieved in 7L fermentor under the optimum conditions and the fermentation time was saved.

关 键 词：赭曲霉 菌球 11α羟基坎利酮 转化率 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]