溶葡球菌酶5升罐发酵研究  

Studies on Lysostaphin Fermentation in 5-Liter Fermentor

作　　者：张培德[1] 吴蓉[1] 陈石根[1] 

机构地区：[1]复旦大学生物化学系,上海200433

出　　处：《工业微生物》1997年第1期13-16,共4页Industrial Microbiology

摘　　要：利用含溶葡球菌酶基因的枯草芽孢杆菌转化子,进行半合成培养基的5升自动发酵罐的中试发酵条件试验,在控制转速（450～500r/min）和通气量（1:0.5～0.8）二个条件下,采用分批培养和补料分批培养,获得了一组在半合成培养基上生产溶葡球菌酶的较优化条件,产酶高峰在8.5h～9h,酶产量达465mg/L。Parameters affecting the lysostaphin production of Bacillus subtilis transfor-mant containing the lysostaphin gene ware optimized in a 5-liter autofermentor with semi-synthetic media. A favorable set of parameters in batch and feed batch culture was established by the adjustment of rotating velocity (450-500r/ min) and air flow rate (1:0.5-0.8). Peak production of the enzyme was at 8.5 to 9 hours after fermentation with a maximum yield of 465 mg/l.

关 键 词：溶葡球菌酶 发酵 半合成培养基 生产 

分 类 号：TQ925.9[轻工技术与工程—发酵工程]