丙酮-硫酸铵双水相体系分离纯化红花红色素  

Separation and purification of carthamine from carthamus tinctorius by acetone-ammonium sulfate aqueous two-phase system

作　　者：卢俊彩[1] 崔岩岩[1] 陈火林[1] 何佳恒[1] 龙浩骑[1] 高强[1] 祝小红[2] 陈光磊[2] 李首建[1] 

机构地区：[1]四川大学化学学院,四川成都610064 [2]四川大学分析测试中心,四川成都610064

出　　处：《化学研究与应用》2007年第6期679-682,共4页Chemical Research and Application

基　　金：四川省科技厅攻关课题;项目编号:06(05SG022-021-04)

摘　　要：A kind of simple aqueous two-phase system composed of acetone,inorganic salt and water was used to separate and purify carthamine from safflower petals.Experimental results show that(NH4)2SO4 has the stronger phases-forming ability than K2HPO4 and NaCl.The optimum conditions for isolating carthamine from safflower yellow were chose as V(CH3)2CO=5mL,VH2O=5mL,m(NH4)2SO4=2.5g.Under the conditions,the yield can reach to 99 % for the extraction of carthamine with the partition coefficient K>55.The carthamine product with high purity was obtained using analog counter-current extracting experiment.A kind of simple aqueous two - phase system composed of acetone, inorganic salt and water was used to separate and purify carthamine from safflower petals. Experimental results show that （NH4 ）2SO4 has the stronger phases -forming ability than K2 HPO4 and NaCl. The optimum conditions for isolating carthamine from safflower yellow were chose as V（ CH3 ）2 CO = 5mL, VH2O =5mL,m（NH4）2SO4 =2.5g. Under the conditions, the yield can reach to 99 % for the extraction of carthamine with the partition coefficient K 〉 55. The earthamine product with high purity was obtained using analog counter - current extracting experiment .

关 键 词：红花 红色素 双水相 

分 类 号：O629.4[理学—有机化学]