可降解淀粉酿酒酵母基因工程菌的构建及其生产应用  

Construction of Amylolytic Yeast Engineered Strains and Its Application

作　　者：程少菊[1] 张添元[1] 罗进贤[1] 吴群悦[1] 

机构地区：[1]中山大学生命科学学院生物化学系//基因工程教育部重点实验室,广东广州510275

出　　处：《中山大学学报（自然科学版）》2007年第3期128-130,共3页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省自然科学基金资助项目(011207)

摘　　要：将酿酒酵母的rDNA片段,黑曲霉葡萄糖淀粉酶基因表达盒及G418抗性基因表达盒重组进经过改造的质粒pSP72,构建酿酒酵母整合型质粒YIp4RGAn及YIp19RGAn,转化酿酒酵母实验室菌株GRF18、生产菌株JL108、SD和JM,获得能高效表达葡萄糖淀粉酶和分解淀粉的酿酒酵母基因工菌。Southern印迹分析证明,葡萄糖淀粉酶基因已整合进工程菌染色体。这些工程菌在含有20%淀粉的培养基中培养,产酒率都在11%以上。Saccharomyces cerevisiae integrated plasmids YIp4RGAn and YIpl9RGAn were constructed by cloning the S. cerevisiae rDNA fragment, Aspergillus niger glucoamylase gene and neo^r gene expression cassettes into the modified plasmid pSP72, and transformed into the laboratory yeast strain GRF18 and ethanol-producing strains JL108, SD and JM resulting in several ethanol-producing yeast engineered strains with efficient expression capability. Southern blot results demonstrated that the glucoamylase gene was integrated into the chromosome of these engineered strains. These engineered strains secrete more than 11% ethanol when they are cultivated in medium containing 20% starch.

关 键 词：降解淀粉 酿酒酵母工程菌 酒精生产 重组DNA技术 

分 类 号：Q78[生物学—分子生物学]