钴60与紫外线复合诱变选育L-鸟氨酸高产菌株  

Screening of L-ornithine-producing mutant by ^(60)Co γ irradation and UV method

作　　者：邹玉玲[1] 薛海燕[2] 汪江波[1] 张婧芳[1] 张小菊[3] 

机构地区：[1]湖北工业大学生物工程学院,湖北武汉430068 [2]台州学院生物工程系,浙江台州318000 [3]华中科技大学武昌分校城市建设系,湖北武汉430068

出　　处：《华西药学杂志》2007年第3期263-265,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的诱变选育L-鸟氨酸高产菌株。方法以谷氨酸棒杆菌S9114为出发菌株,经60Coγ射线和紫外线复合诱变处理,选育出L-鸟氨酸的高产菌株,研究其最佳发酵条件。结果选育出一株L-鸟氨酸的高产菌株HG106-25,遗传标记为精氨酸营养缺陷型+磺胺胍抗性(Arg-+SGr)。结论突变株HG106-25在优化发酵条件后,摇瓶发酵4~5 d,L-鸟氨酸平均产量为14.7 g.L-1,达较高水平,可进行放大试验。OBJECTIVE To breed a L - omithine - producing mutant. METHODS An L - omithine - producing mutant was obtained from Glutamicum corymebacterium $9114 by treatment with ^60 Co γ irradation and ultraviolet. RESULTS A L -ornithine - producing mutant of HG106 - 25 （ Arg - ＋ SGr ） was selected, which didn＇ t grow without L - Arg and resisted to SG. CONCLUSION After optimization of the fermentation conditions, the strain could accumulate an average yield of 14.7 g·L^-1 L - omithine in the medium, when it was cultured at 31 ℃ and 150 r·min^-1 in a constant temperature shaker for 4 -5 days under the optimized culturing conditions. So the strain can be further studied to be sealed up in fermenter.

关 键 词：L-鸟氨酸 谷氨酸棒杆菌 诱变 发酵 

分 类 号：TQ922[轻工技术与工程—发酵工程]