食管鳞状上皮细胞癌中P53基因突变的PCR-SSCP检测  

The Detection on Mutations of P53 Gene in Esophageal Squamous Cell Carcinomas by PCR-SSCP Analysis

作　　者：王江[1] 孟振行[2] 张三申 李新荣[1] 郭山春[2] 张云汉[1] 

机构地区：[1]河南医科大学病理学教研室 [2]北京医科大学病理学系 [3]河南省安阳市肿瘤医院病理科

出　　处：《河南肿瘤学杂志》1997年第2期81-83,共3页Henan Journal of Oncology

摘　　要：应用PCR－SSCP银染技术检测了40例食管鳞状细胞癌p53基因第5、6、7和第8外显于的突变情况。结果显示：13例（32．5％）有p53基因突变，突变主要发生在第5和第8外显子，与癌分化程度无明显关系，但伴淋巴结癌转移病例中p53基因的突变率明显高于无转移者（P＜0．05）。本研究提示，p53基因突变存在于中、晚期食管癌中，并可能与食管鳞癌的进展与转移有关。The mutations within exon 5,6,7 and 8 of p53 gene were dethetal in fortyesophageal squamors cellcarcmomas by analysis of PCR single-srand conformation polymorphisms(SSCP). The results showed thatthlrteen Samples contained p53 mutations (32 .5% ). The mutations were foun pedominantly within exon5 and exon 8. The incidenCe of mutation of P53 gene in the cases of lymPh nede metastasis was obviouslyhigher than that in Patients with out metastasis (p<0.05). Thepresent study sugests that the mutationof p53 gene may be involved in the middle and advanced stages of esophageal carcinomas and related withthe progress and metastasis of esopageal squamous cell carcinioma.

关 键 词：PCR-SSCP 食管肿瘤 鳞癌 P53基因 基因突变 

分 类 号：R735.102[医药卫生—肿瘤]