p57^(kip2) mRNA在肝细胞癌发生过程中的表达  

Expression of p57^(kip2) mRNA in Human Hepatocarcinogenesis

作　　者：孔萌萌[1] 金克炜[2] 申丽娟[2] 章宗籍[2] 张华献[2] 孙江华[2] 

机构地区：[1]同济大学生命科学与技术学院肿瘤研究所,上海200092 [2]昆明医学院病理教研室,云南昆明650031

出　　处：《昆明医学院学报》2007年第3期10-12,24,共4页Journal of Kunming Medical College

基　　金：云南省自然科学基金资助(2000C0058M;2005C0045M)

摘　　要：目的研究p57kip2 mRNA在人体肝细胞癌发生不同阶段的表达状况,探讨与肝癌发生发展及分化的关系.方法采用原位分子杂交技术检测正常肝组织、慢性肝炎、肝硬化、癌周肝硬化和肝细胞癌共42例标本中p57kip2 mRNA的表达.结果正常肝组织组未见p57kip2 mRNA表达,慢性肝炎组阳性表达率为57.14%(4/7),肝硬化组阳性表达率为40.00%(4/10),癌周肝硬化组与肝癌组阳性表达率分别为70.00%(7/10)和20.00%(2/10).肝癌组p57kip2 mRNA阳性表达率明显下降,与癌周肝硬化组差异有统计学意义(P<0.05).肝癌组分化好者与分化差者表达差异有统计学意义(P<0.05).结论p57kip2 mRNA表达下降在原发性肝癌的发生发展中起着重要作用,且可能与肝癌的分化有关.Objective To study the expression of p57^kip2 mRNA during different phases genesis and to explore their relationships with the occurrence, development and differentiation of Methods In situ hybridization （ISH） for p57^kip2 mRNA was performed in formalin -fixed and of hepatocarcino- hepatocarcinoma. paraffin - embedded sections in 88 cases of normal liver tissue （n = 10）, chronic hepatitis （n = 18）, liver cirrhosis （n =20）, paracancerous cirrhosis （n = 18） and hepatocellular carcinoma （n = 22）. Results The positive rate of p57^kip2 mRNA was 57. 14% （4/7） in chronic hepatitis, 40. 00% （4/10） in liver cirrhosis, 70. 00% （7/10） in paratumor cirrhosis and 20. 00% （2/10） in hepatocellular carcinoma respectively. The expression of p57^kip2 mRNA in paratumor cirrhosis was significantly higher than that in hepatocellular carcinoma （ P 〈 0. 05 ）. And there was statistical difference of the expression of p57^kip2 mRNA between well differentiated hepatocellular carcinoma and that of poorly differentiated hepatocellular carcinoma （P 〈 0. 05）. Conclusion The decrease of p57^kip2 mRNA in hepatocellular carcinoma indicates that p57^kip2 may be involved in human hepatocarcinogenesis and its differentiation.

关 键 词：肝细胞癌 肝炎 肝硬化 P57^KIP2 原位杂交 

分 类 号：R735.7[医药卫生—肿瘤]