超临界流体萃取-反相高效液相色谱法测定玉米蛋白粉中叶黄质和玉米黄质的含量  

Determination of the Lutein and Zeaxanthin in Corn Gluten Meal Extract by SFE-RP-HPLC

作　　者：綦丹华[1] 卢怀宝[1] 姚双[1] 马玲玲[2] 杨杰[1] 程晓凤[1] 姚渭溪[2] 

机构地区：[1]大庆石油管理局,黑龙江大庆163453 [2]中国科学院生态环境研究中心,北京100085

出　　处：《天津师范大学学报（自然科学版）》2007年第2期1-3,6,共4页Journal of Tianjin Normal University：Natural Science Edition

基　　金：大庆石油管理局资助科研项目(0007009)

摘　　要：研究了超临界流体萃取（SFE）玉米蛋白粉中类胡萝卜素的提取工艺及其收率,结果显示：当45℃,26 MPa,改性剂体积分数为10%-20%,CO2流量为80-120 L/h,动态提取3 h时,类胡萝卜素的收率达到9%;用反相高效液相色谱法（RP-HPLC）测定了3种单体胡萝卜素（叶黄质、玉米黄质和β-胡萝卜素）的含量,其中叶黄质0.023%,玉米黄质0.004 2%,β-胡萝卜素0.0015%.同时还与万寿菊提取物中叶黄质含量（0.068%）进行了比较.The extractive technology and yield of the lutein, zeaxanthin and β-carotene from corn gluten meal extract were carried out by supercritical fluid extraction （SFE）. The extractive rate of carotenoid was 9 % when extractive temperature was 45℃ and extractive pressure was 26 MPa, the volume fraction of modifier 10%-20%, flux of carbon dioxide 80- 120 L/h and dynamic time of extraction 3 h. Three carotenoid monomers in the extracts were determined with RP-HPLC. The contents of lutein, zeaxanthin and β-carotene were 0. 023%, 0. 004 2% and 0. 001 5% respectively. Content of lutein was simultaneously compared with marigold extract, which content of lutein is 0. 068 %.

关 键 词：高效液相色谱法 叶黄质 玉米黄质 Β-胡萝卜素 玉米蛋白粉 超临界流体萃取 

分 类 号：TQ041.7[化学工程]