小檗碱在预防家兔颈动脉粥样硬化中对血管紧张素Ⅱ1型受体的影响  

The Effect of Berberine on the Angiotensin Ⅱ Receptor Type 1 in the Prevention of Carotid Artery Atherosclerosis in Rabbits

作　　者：何国厚[1] 周岚[1] 王云甫[1] 艾志兵[1] 

机构地区：[1]郧阳医学院附属太和医院神经内科,湖北省十堰市442000

出　　处：《中国动脉硬化杂志》2006年第12期1035-1037,共3页Chinese Journal of Arteriosclerosis

摘　　要：目的探讨小檗碱在预防家兔颈动脉粥样硬化中对血管紧张素Ⅱ1型受体的影响。方法32只雄性新西兰大白兔随机分成正常组、颈动脉粥样硬化组(模型组)、生理盐水组(对照组)、小檗碱预防组(小檗碱组)。除正常组外,其余各组建立颈动脉粥样硬化模型,术前1周分别给予对照组和小檗碱组肌注生理盐水和小檗碱,术后4周内继续进行上述干预。第5周麻醉处死后取右侧颈动脉,行HE染色和血管紧张素Ⅱ1型受体免疫组织化学染色。结果HE染色发现模型组颈动脉血管病变以泡沫细胞为主,形成动脉粥样斑块;对照组病理改变与模型组类似;小檗碱组血管内膜轻度增厚。颈动脉血管紧张素Ⅱ1型受体免疫组织化学染色发现,模型组和对照组颈动脉粥样硬化组织中平滑肌细胞和泡沫细胞胞膜血管紧张素Ⅱ1型受体表达丰富,阳性细胞数密度较正常组明显增高;小檗碱组血管中膜平滑肌细胞仅有少量血管紧张素Ⅱ1型受体表达,血管紧张素Ⅱ1型受体阳性细胞数密度明显低于对照组(P<0.05)。结论小檗碱预防颈动脉粥样硬化的形成可能与其对颈动脉组织中血管紧张素Ⅱ1型受体表达的调节有关。Aim To explore the effect of berberine on the angiotensin Ⅱ（AngⅡ） receptor type 1（AT1R） in the prevention of carotid artery atherosclerosis in rabbits. Methods Thirty-two New Zealand white rabbits were divided into four groups： normal group, carotid artery atherosderosis （model group） group, normal saline group （control group） and berberine prevention group （berberine group）. Except normal group with normal diet, other groups were fed with high cholesterol-diet and airdried on the common carotid, on the basis of that, physiological saline group and berberine group were treated with intramuscular physiological saline injection and berberine respectively. After air-dry for four weeks, all rabbits were sacrificed with anaesthesia. The right carotid arteries were also harvested for HE staining and immunohistochemistry of AT1R after the sacrifice. Results The vaseulature structure of the carotid arteries was characterized by foam ceils which formed atheromatous plapue in model group, and thickening in intima with few foam cells underneath in berbefine group. There were few positive cells in media of the common carotid in normal group and the expression of AT1R was found both in model group and control group, and the former was much lower. The number density of the positive ceils in berberine group were decreased compared with that in control group （ P 〈 0.05 ）. Conclusions The mechanism of preventing development of carotid artery atherosclerosis for berberine may be through inhibiting expression of AT1R.

关 键 词：病理学与病理生理学 小檗碱 颈动脉粥样硬化 血管紧张素Ⅱ1型受体 阳性细胞数 

分 类 号：R363[医药卫生—病理学]